Elovl5

Manufactured by Thermo Fisher Scientific

Sourced in United States

ELOVL5 is a laboratory equipment product that functions as an elongase enzyme involved in the biosynthesis of long-chain and very long-chain fatty acids. The core function of ELOVL5 is to catalyze the rate-limiting condensation step in the elongation of fatty acids.

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Lab products found in correlation

Ar v7, by Thermo Fisher Scientific (2 mentions) Anti ar, by Merck Group (2 mentions) Mboat2, by Thermo Fisher Scientific (2 mentions) Elovl7, by Thermo Fisher Scientific (2 mentions) Tmprss2, by Thermo Fisher Scientific (2 mentions) Anti elovl7, by Abcam (2 mentions) Acsl3, by Thermo Fisher Scientific (2 mentions) Anti ar v7, by Abcam (2 mentions) Anti β actin, by Abcam (2 mentions) Anti gapdh, by Abcam (2 mentions)

4 protocols using elovl5

Primer Sequences for ChIP-qPCR and RT-qPCR Analyses

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For ChIP-qPCR, primers for KLK3-enh, KLK2-enh, NKX3.1-enh, SGK1-enh, SGK3-enh, MBOAT2-enh, RAB11B-FBS, PDK4-FBS, and CDK1-FBS were listed in Supplementary Table 1. Primers for TMPRSS2-enh, ZBTB16-enh, and SH2B1-enh were described previously 11 (link). Primers for TFF1-enh, NRIP1-enh, PGR-enh, and DSCAM-enh were described previously 56 (link),57 (link).
For RT-qPCR, primer and probe sets for KLK3 and ZBTB16 were listed in Supplementary Table 1. Primer and probe sets for MBOAT2 (Hs01027245_m1), ELOVL7 (Hs00405151_m1), FKBP5 (Hs01561006_m1), NKX3.1 (Hs00171834_m1), SGK1 (Hs00178612_m1), ACSL3 (Hs00244853_m1), ELOVL5 (Hs01094711_m1), NANS (Hs00219054_m1), and AR-V7 (AI6R0CI) were purchased from Thermo Fisher.

Gao S., Chen S., Han D., Wang Z., Li M., Han W., Besschetnova A., Liu M., Zhou F., Barrett D., Luong M.P., Owiredu J., Liang Y., Ahmed M., Petricca J., Patalano S., Macoska J.A., Corey E., Chen S., Balk S.P., He H.H, & Cai C. (2020). Chromatin binding of FOXA1 is promoted by LSD1-mediated demethylation in prostate cancer. Nature genetics, 52(10), 1011-1017.

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Primer Sequences for ChIP-qPCR and RT-qPCR Analyses

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Quantitative RT-PCR and Immunoblotting Protocol

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The mRNA was measured using real-time RT-PCR with Taqman one-step RT-PCR reagents and results were normalized to co-amplified GAPDH. The primers and probes are listed as following: ACSL3 (Hs00244853_m1), MBOAT2 (Hs01027245_m1), ELOVL5 (Hs01094711_m1), ELOVL7 (Hs00405151_m1), SC5D (Hs00999007_m1), NANS (Hs00219054_m1), TMPRSS2 (Hs01120965_m1), NKX3.1 (Hs00171834_m1) (purchased from Applied Biosystems); PSA: forward, 5′-GATGAAACAGGCTGTGCCG-3′, reverse, 5′-CCTCACAGCTACCCACTGCA-3′, probe, 5′-FAM-CAGGAACAAAAGCGTGATCTTGCTGGG-3′; AR-V7: forward: 5′-CGGAAATGTTATGAAGCAGGGATGA-3′, reverse, 5′-CTGGTCATTTTGAGATGCTTGCAAT-3′, probe, 5′-FAM-GGAGAAAAATTCCGGGT-3′. For immunoblotting, cells were lysed with RIPA buffer with protease inhibitors and anti-Ser240/244 phosphorylated S6 (Cell Signaling), anti-AR (Millipore), anti-AR-V7, anti-ELOVL7, anti-β-actin, anti-GAPDH (Abcam), or anti-β-tublin (Upstate) antibodies were used. Gels shown are representative of at least 3 independent experiments.

Han W., Gao S., Barrett D., Ahmed M., Han D., Macoska J.A., He H.H, & Cai C. (2017). Reactivation of androgen receptor-regulated lipid biosynthesis drives the progression of castration-resistant prostate cancer. Oncogene, 37(6), 710-721.

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Quantification of mRNA Expression Using RT-PCR

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The mRNA was measured using real-time RT-PCR with Taqman one-step RT-PCR reagents and results were normalized to co-amplified GAPDH. The primers and probes are listed as following: ACSL3 (Hs00244853_m1), MBOAT2 (Hs01027245_m1), ELOVL5 (Hs01094711_m1), ELOVL7 (Hs00405151_m1), SC5D (Hs00999007_m1), NANS (Hs00219054_m1), TMPRSS2 (Hs01120965_m1), NKX3.1 (Hs00171834_m1) (purchased from Applied Biosystems, Foster City, CA, USA); PSA: forward, 5′-GATGAAACAGGCTGTGCCG-3′, reverse, 5′-CCTCACAGCTACCCACTGCA-3′, probe, 5′-FAM-CAGGAACAAAAGCGTGATCTTGCTGGG-3′ AR-V7: forward: 5′-CGGAAATGTTATGAAGCAGGGATGA-3′, reverse, 5′-CTGGTCATTTTGAGATGCTTGCAAT-3′, probe, 5′-FAM-GGAGAAAAATTCCGGGT-3′. For immunoblotting, cells were lysed with RIPA buffer with protease inhibitors and anti-Ser240/244 phosphorylated S6 (Cell Signaling, Danvers, MA, USA), anti-AR (Millipore, Billerica, MA, USA), anti-AR-V7, anti-ELOVL7, anti-β-actin, anti-GAPDH (Abcam, Cambridge, MA, USA), or anti-β-tubulin (Upstate, Billerica, MA, USA) antibodies were used. Gels shown are representative of at least 3 independent experiments.

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