Mice (n = 6 in each group) were decapitated, the hippocampus were immediately dissected, frozen and stored at −80 °C. Tissue samples were lysed and western blots were performed as previously described [11 (link)]. Primary antibodies include GFP (1:1000, Beyotime, cat # AG281, Shanghai, China), Glial fibrillary acidic protein (GFAP) (1:1000, Wanleibio, cat # WL0836, Shenyang, China), Flag (1:1000, abm, cat # G188, Zhenjiang, China), Oligo2 (1:1000, Wanleibio cat # WL04942, Shenyang, China), NeuN (1:1000, Wanleibio, cat # WL03099, Shenyang, China), doublecortin (Dcx) (1:1000, Proteintech, cat # 13925-1-AP, Rosemont, USA), GAPDH (1:5000, Boster, cat # BM1623, Wuhan, China) and β-Tubulin (1:5000, Beidibio, Nanjing, China). HRP-conjugated secondary antibodies purchased from Immunoway Biotechnology Company (goat anti mouse cat # RS0001 and goat anti rabbit cat # RS0002, Plano, TX, USA) were used to probe these blots. Protein visualization was carried out using an enhanced chemiluminescen (ECL, Meilunbio, Dalian, China). The signal intensity was obtained by densitometric scanning.
Hrp conjugated secondary antibodies
Manufactured by Immunoway
Sourced in United States
HRP-conjugated secondary antibodies are laboratory reagents used in immunoassays and other immunochemical techniques. They consist of a secondary antibody that is covalently linked to the enzyme horseradish peroxidase (HRP). The HRP enzyme can catalyze a colorimetric or chemiluminescent reaction, which allows for the detection and quantification of target analytes in a sample.
Automatically generated - may contain errors
Lab products found in correlation
Gapdh, by Boster Bio (1 mentions)
Glial fibrillary acidic protein (gfap), by Wanlei (1 mentions)
Flag tag (flag), by Applied Biological Materials (1 mentions)
Enhanced chemi luminescence (ecl), by Meilun (1 mentions)
Chemiluminescence system, by Merck Group (1 mentions)
Anti rpa2, by Abcam (1 mentions)
Anti flag tag, by Cell Signaling Technology (1 mentions)
Anti rad51, by Abcam (1 mentions)
Anti β tubulin, by Immunoway (1 mentions)
Anti primpol, by Abcam (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
2 protocols using hrp conjugated secondary antibodies
1
Hippocampal Protein Analysis in Mice
2
Western Blot Analysis of Cellular Proteins
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Cell extracts were harvested and separated by SDS-PAGE, and then transferred to PVDF membranes (Millipore, Billerica, USA). The membranes were blocked with 5% non-fat milk for 1 h at room temperature, washed, and incubated with specific antibodies overnight at 4°C. The membranes were washed again and incubated with the corresponding HRP-conjugated secondary antibodies (Immunoway). Finally, protein bands were detected using a chemiluminescence system (Millipore). The primary antibodies used are as follows: anti-FLAG-tag (Cell Signaling Technology), anti-RPA2 (Abcam), anti-β-tubulin (Immunoway), anti-PRIMPOL (Abcam), and anti-RAD51 (Abcam).
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