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Infinite 200 pro plate

Manufactured by Tecan
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The Infinite 200 PRO is a multimode microplate reader designed for a variety of absorbance, fluorescence, and luminescence detection applications. It provides high-performance detection capabilities for precise and reproducible measurements.

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Lab products found in correlation

Luminol, by Merck Group (1 mentions) 400 mesh carbon coated copper grid, by Ted Pella (1 mentions) Dynapro plate reader 2, by Wyatt Technology (1 mentions) Human cardiac troponin 1 elisa kit, by Abcam (1 mentions) Multimode afm, by Bruker (1 mentions) Mica substrates, by Electron Microscopy Sciences (1 mentions) Daf fm, by Merck Group (1 mentions) Tnf α, by BioLegend (1 mentions)

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Infinite 200 PRO (2479 citations) Infinite 200 (774 citations) Infinite 200 PRO plate reader (410 citations) Infinite® 200 PRO NanoQuant plate reader (10 citations) Infinite 200 PRO multimode plate reader (9 citations) Infinite 200 Pro M Nano plate reader (4 citations) TECAN 200 Pro (2 citations) Infinite® 200 PRO series plate (2 citations) Infinite® 200 PRO microtiter plate reader (2 citations) Infinite 200 PRO microplate plate reader (2 citations)

15 protocols using infinite 200 pro plate

1

Luminol-based Assay for Neutrophil ROS

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Reactive oxygen species generation was assessed by luminol-amplified chemiluminescence as previously described (25 (link)). Briefly, resting neutrophils [1 × 105 in HBSS+/+ (+calcium, +magnesium)] were dispensed into a 96-well white-bottom flat plate (Corning, United States) containing 1 μM luminol (pH 7.3; Sigma-Aldrich). Cells were stimulated with 25 nM PMA (test) or HBSS+/+ and immediately assessed for ROS generation at 1-min intervals for 60 min using a Tecan Infinite 200 Pro plate reader (Tecan, Mannedorf, Switzerland). Experiments were performed in triplicate, with ROS production measured as relative light units (RLU) and calculated as area under the curve (AUC).
Bartlett D.B., Slentz C.A., Willis L.H., Hoselton A., Huebner J.L., Kraus V.B., Moss J., Muehlbauer M.J., Spielmann G., Muoio D.M., Koves T.R., Wu H., Huffman K.M., Lord J.M, & Kraus W.E. (2020). Rejuvenation of Neutrophil Functions in Association With Reduced Diabetes Risk Following Ten Weeks of Low-Volume High Intensity Interval Walking in Older Adults With Prediabetes – A Pilot Study. Frontiers in Immunology, 11, 729.
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2

Measuring Cellular Oxidative Stress

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Cells were incubated with 100 μM DCF-diacetate diluted in PBS post treatment for 30 min at 37 °C and 5 % CO2. Afterwards, the DCF-diacetate dilution was removed and the fluorescence at 485ex/535em nm was measured using the Infinite 200 PRO plate-reader (Tecan).
Vogeley C., Sondermann N.C., Woeste S., Momin A.A., Gilardino V., Hartung F., Heinen M., Maaß S.K., Mescher M., Pollet M., Rolfes K.M., Vogel C.F., Rossi A., Lang D., Arold S.T., Nakamura M, & Haarmann-Stemmann T. (2021). Unraveling the differential impact of PAHs and dioxin-like compounds on AKR1C3 reveals the EGFR extracellular domain as a critical determinant of the AHR response. Environment international, 158, 106989.
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3

Cell Viability Assay using MTT

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Cells (5000 per well) were seeded in 96-well plates in phenol-red-free medium supplemented with charcoal-stripped FBS. Treatments were initiated after the cells were attached. At the appropriate time points, cell viability was determined by MTT assay; 10 μl of MTT (5mg/ml in phosphate-buffered saline) was added to each well followed by incubation at 37°C for 2 hours. The formazan crystal sediments were dissolved in 100 μl of dimethyl sulfoxide and absorbance was measured at 590 nm using the Tecan Infinite 200 Pro-plate reader. Each treatment was performed in seven replicate wells and repeated three times.
McFall T., Diedrich J.K., Mengistu M., Littlechild S.L., Paskvan K.V., Sisk-Hackworth L., Moresco J.J., Shaw A.S, & Stites E.C. (2019). A systems mechanism for KRAS mutant allele-specific responses to targeted therapy. Science signaling, 12(600), eaaw8288.
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4

Static Biofilm Formation Assay

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Static biofilm formation was tested as previously described (Marroquin et al. 2019 (link)). Overnight bacterial cultures were grown in BHI then diluted 1:50 into a 96-well plate containing 10% human plasma. Plates were incubated at 37°C for 24 h. Following incubation, plates were washed two times with sterile PBS and stained with .05% crystal violet for 5 minutes at 60°C. Resulting biofilms were washed two more times with PBS and the remaining adherent cells treated with 30% acetic acid. Biomass was quantified by OD595 in a Tecan Infinite 200pro plate reader.
Keogh R.A., Huyvaert S., Moore G.D., Horswill A.R, & Doran K.S. (2024). Virulence characteristics of Gram-positive bacteria isolated from diabetic foot ulcers. FEMS Microbes, 5, xtae013.
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5

Measuring Cellular Oxidative Stress

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Cells were incubated with 100 μM DCF-diacetate diluted in PBS post treatment for 30 min at 37 °C and 5 % CO2. Afterwards, the DCF-diacetate dilution was removed and the fluorescence at 485ex/535em nm was measured using the Infinite 200 PRO plate-reader (Tecan).
Vogeley C., Sondermann N.C., Woeste S., Momin A.A., Gilardino V., Hartung F., Heinen M., Maaß S.K., Mescher M., Pollet M., Rolfes K.M., Vogel C.F., Rossi A., Lang D., Arold S.T., Nakamura M, & Haarmann-Stemmann T. (2021). Unraveling the differential impact of PAHs and dioxin-like compounds on AKR1C3 reveals the EGFR extracellular domain as a critical determinant of the AHR response. Environment international, 158, 106989.
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6

Characterizing Colloidal Nanoparticle Properties

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Diameter, polydispersity, and normalized scattering intensity were measured using dynamic light scattering (DLS). A DynaPro Plate Reader II (Wyatt Technologies), with a laser width optimized for colloidal aggregate detection by the manufacturer, was used with a 60 mW laser at 830 nm wavelength and a detector angle of 158°. Fluorescence intensity of colloids coformulated with the BOPDIY dye FRET pair was measured using the Tecan Infinite 200 Pro plate reader. The FRET pair was excited at 490 nm, and the acceptor emission was measured at 575 nm.
Morphology of colloids was assessed by transmission electron microscopy where 5 μL of colloid solution was deposited on glow-discharged 400 mesh carbon-coated copper grids (Ted Pella Inc.) and allowed to adhere for 3 min. Excess liquid was removed and grids washed with 5 μL of double-distilled water. Grids were stained with 1% ammonium molybdate (pH 7, 5 μL) for 30 s. After excess stain removal, samples were imaged using a Talos L120C transmission electron microscope operating at 80 kV. Images were captured using a CETA CMOS camera and analyzed using ImageJ software.
Ganesh A.N., Aman A., Logie J., Barthel B.L., Cogan P., Al-awar R., Koch T.H., Shoichet B.K, & Shoichet M.S. (2019). Colloidal Drug Aggregate Stability in High Serum Conditions and Pharmacokinetic Consequence. ACS chemical biology, 14(4), 751-757.
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7

ELISA-based Biomarker Detection Protocol

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ELISA-based assays were performed using kits for lactate dehydrogenase (LDH), troponin T, and CK-MB from InvivoGen (San Diego, CA, USA). Colorimetric and luminescent signals from ELISA kits were measured using a Tecan Infinite 200 PRO plate reader (Männedorf, Switzerland), according to the manufacturer’s instructions [55 (link),56 ].
Wang Y., Jasper H., Toan S., Muid D., Chang X, & Zhou H. (2021). Mitophagy coordinates the mitochondrial unfolded protein response to attenuate inflammation-mediated myocardial injury. Redox Biology, 45, 102049.
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8

Growth Curves and Antibiotic Sensitivity of Streptococcus suis

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Growth curves were conducted by dilution of overnight cultures to an OD600 of 0.1. These cultures were then used to seed a 96-well plate in triplicate. Plates were then incubated at 37°C with shaking in a Tecan Infinite 200 Pro plate reader, with absorbance readings (OD600) being taken every 15 min.
The MIC was measured by broth microdilution in triplicate experiments based on CLSI guidelines as described previously (51 (link)). Briefly, an overnight culture of S. suis was diluted to an OD600 of 0.1 and subcultured grown to the mid-log phase for 3 h at 37°C. Mid-log-phase cultures were diluted to an OD600 of 0.2, and 50 μl of each culture was added to 96-well plates containing serially diluted antibiotic concentrations (5 to 0.08 μg/ml), and plates were grown at 37°C with 5% CO2 for 24 h. The MIC (mg/liter) was determined as the last dilution at which turbidity was observed following overnight growth, with all assays being performed in triplicate.
Tram G., Jen F.E., Phillips Z.N., Timms J., Husna A.U., Jennings M.P., Blackall P.J, & Atack J.M. (2021). Streptococcus suis Encodes Multiple Allelic Variants of a Phase-Variable Type III DNA Methyltransferase, ModS, That Control Distinct Phasevarions. mSphere, 6(3), e00069-21.
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9

hiPSC-CM Troponin-I Release Assay

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Troponin-I release was measured from hiPSC-cardiomyocytes seeded in monolayer onto vitronectin-coated CellCarrier-96 well plates. Compounds were added to the culture 10 days after seeding, and were left without refreshing for 4 consecutive days. Then, media of two culture wells with the same condition were pooled and stored at -20°C until measurement. Free TnI was quantified using the Human Cardiac Troponin I ELISA kit (Abcam) according to manufacturer’s instructions. Troponin quantity was determined using a Tecan Infinite 200 PRO plate reader using spectrometry at OD 450 nm.
Schwach V., Slaats R.H., Cofiño-Fabres C., ten Den S.A., Rivera-Arbeláez J.M., Dannenberg M., van Boheemen C., Ribeiro M.C., van der Zanden S.Y., Nollet E.E., van der Velden J., Neefjes J., Cao L, & Passier R. (2024). A safety screening platform for individualized cardiotoxicity assessment. iScience, 27(3), 109139.
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10

Quantitative RAS Activation Assay

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The RAS G-LISA assay was performed using the RAS G-LISA activation kit (catalog BK131; Cytoskeleton) according to the manufacturer’s instructions. Protein (20 μg) from whole-cell lysates was added in triplicate in a 96-well plate, and activated RAS was bound to a RAS–GTP binding protein linked to each well. Bound, active RAS was detected with an RAS-specific Ab and quantified by measuring the relative absorbance at 490 nm with a Tecan Infinite 200Pro plate reader.
Li T., Kikuchi O., Zhou J., Wang Y., Pokharel B., Bastl K., Gokhale P., Knott A., Zhang Y., Doench J.G., Ho Z.V., Catenacci D.V, & Bass A.J. (2023). Developing SHP2-based combination therapy for KRAS-amplified cancer. JCI Insight, 8(3), e152714.
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