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Ftir frontier spectrometer

Manufactured by PerkinElmer
Sourced in France, United States

The PerkinElmer FTIR (Frontier) spectrometer is a Fourier transform infrared spectrometer. It is designed to measure the infrared spectrum of a sample, providing information about its molecular composition and structure.

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5 protocols using ftir frontier spectrometer

1

Characterization of C-dots Conjugates

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The synthesized conjugates (20 µg mL−1) were tested with UV-vis spectroscopy in a 1 cm quartz cell by using Shimadzu UV-2600 spectrometer. Then the fluorescent emission spectra of the same samples were recorded by Horiba Jobin Yvon Fluorolog-3 with slit width 5 nm for both excitation and emission. The solid FTIR study was performed on a PerkinElmer FTIR (Frontier) spectrometer using the attenuated total reflection (ATR) technique which uses the ATR prism alone as the background. The matrix-assisted laser desorption ionization time of flight (MALDI-TOF) was measured by a Bruker autoflex speed spectrometer. Transmission electron microscopic (TEM) studies were conducted with the JEOL 1200X TEM and atomic force microscopic (AFM) studies were done by an Agilent 5420 atomic force microscope using the tapping mode. Each characterization study was repeated with different batches of C-dots-conjugates to verify the consistency of the data and the stability of the complexes.
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2

Comprehensive Spectroscopic Characterization of CDs

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The UV/Vis absorption spectra of three CDs fractions were obtained by using a Cary 100 UV/Vis spectrophotometer and a 1cm optical cell. A Fluorolog (Horiba Jobin Yvon) spectrometer was used to record the fluorescence emission spectra of sample with a slit width of 5 nm used for both excitation and emission. As for the determination of fluorescence quantum yield, a Varian Cary Eclipse spectrometer was used to record the fluorescence spectra of samples and standards. Fourier-transform infrared (FTIR) spectroscopy data were obtained with a PerkinElmer FTIR (Frontier) spectrometer by using the attenuated total reflection (ATR) technique with air as background. XPS data was acquired using a Perkin-Elmer PHI 560 system with a double-pass cylindrical mirror analyzer operated using a Mg Kα anode with a hʋ = 1253.6 eV photon energy operated at 250 Watts and 13 kV. Zeta potential measurements were made by using a nano series Malvern Zetasizer. AFM images were obtained with an Agilent 5420 atomic force microscope by using the tapping mode. TEM was performed by using a JEOL 1200X TEM.
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3

FTIR Imaging of Tissue Sections

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The IR acquisitions were performed with a tandem device combining a Spotlight™ 400 microscope and a FTIR Frontier™ spectrometer (Perkin Elmer®, Courtaboeuf, France). The detector is composed of a 16 pixel matrix. Each pixel can be considered as an individual IR detector, with a spectrum associated with it. The pixel size on the sample was 6.25 × 6.25 μm2 and the size of tissue sections was approximately 2–3 mm2 which corresponds to 50 000–80 000 spectra/images. Spectra were collected in the wavenumber range from 750 to 4000 cm–1, with a spectral resolution of 4 cm–1 and 8 accumulations/measurements. For each sample, a paraffin image was collected on an area located at the periphery of the tissue with the same parameters. The backgrounds were recorded on a clean area of CaF2 substrates with 32 accumulations/measurements.
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4

FTIR Analysis of PLGA Fiber Surface

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The surface chemistry of the fibers was investigated with a FTIR-Frontier spectrometer (Perkin Elmer Inc., Shelton, USA). PLGA fibers with different surface modifications were encapsulated in the KBr pellet and scanned within a range of 200–4000 cm−1.
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5

Synthesis and Characterization of Photochromic Metal-Organic Framework

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Materials and characterization. Solvents and reagents, including K2[Pt(CN)4]•3H2O, Fe(BF4)•6H2O, trans-azobenzene, trans-stilbene and cis-stilbene, were purchased form Sigma-Aldrich and used without further purification. The preparation of the bpac ligand and the host framework {Fe(bpac)[Pt(CN)4]}•1.5H2O•0.5bpac was carried out according to Ref. [8e,15]. Elemental analysis for CHN were performed after combustion at 850 °C, using IR detection and gravimetry, by means of a Perkin-Elmer 2400 series II device. Mid-IR spectra of the powders were acquired between 4000 -600 cm -1 in ATR mode using a Perkin Elmer FTIR Frontier spectrometer. UV-VIS spectra of the solutions were acquired between 800 -200 nm in transmission mode using a Cary 60 spectrophotometer. Irradiation of the stilbene solution was carried out using a 150 W xenon light source and a UV bandpass filter (275-375 nm). Thermogravimetry (TG) measurements were performed in a nitrogen flow at a heating rate of 5 Kmin -1 in a Netzsch STA 409 C analyzer. Powder X-ray diffraction (PXRD) patterns of the samples have been collected using a Panalytical MPD XPertPro diffractometer [CuKα1 source, Ge(111) monochromator, X'Celerator detector] within the 2θ range 2-60°.
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