Heart tissues were sliced into 4 μm sections, which were then paraffin embedded. After conventional dewaxing to water, the sections underwent microwave heat repair with EDTA sodium citrate antigen repair solution. After incubation with 3% hydrogen peroxide solution at 37 °C in the dark for 10 minutes, the sections were washed with PBS and incubated with 5% BSA at room temperature for 30 minutes. The tissues were incubated with antibodies against CD31 (Abcam, ab281583, 1:500 dilution, UK) and endothelial nitric oxide synthase (eNOS) (Servicebio, GB12086, 1:500 dilution, China) overnight at 4 °C. After being washed with PBS, the tissues were incubated with Alexa Fluor 488 (Servicebio, GB25303, 1:500 dilution, China) and coralite594 (Proteintech, SA00013-3-100, 1:300 dilution, China) for 1 h at room temperature. Images were captured with an inverted laser confocal microscope (ZEISS, LSM710, Germany).
Gb25303
Manufactured by Proteintech
Sourced in China
GB25303 is a DNA extraction kit produced by Proteintech. It is designed for the rapid and efficient extraction of high-quality genomic DNA from a variety of sample types, including plant, animal, and bacterial cells. The kit includes all necessary reagents and buffers to facilitate the DNA extraction process.
Automatically generated - may contain errors
Lab products found in correlation
Lsm 710, by Zeiss (2 mentions)
Ab281583, by Abcam (1 mentions)
Inverted fluorescence microscope, by Leica (1 mentions)
Coralite594, by Proteintech (1 mentions)
Occludin, by Wuhan Servicebio Technology (1 mentions)
Gb111402, by Wuhan Servicebio Technology (1 mentions)
Alexa fluor 488, by Wuhan Servicebio Technology (1 mentions)
2 protocols using gb25303
1
Immunofluorescence Analysis of Endothelial Markers
2
Immunofluorescence Imaging of Ileum Tight Junctions
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For immunofluorescence (IF), the ileum tissue samples were fixed in 4% paraformaldehyde, and then sectioned. The sections were repaired by microwave heating with 0.01 M sodium citrate antigen retrieval buffer. After incubated with hydrogen peroxide solution, the sections were sealed with 5% BSA at room temperature for 30 min. Then the sections were incubated with ZO-1 (Servicebio, GB111402, 1:500 dilution, China) and Occludin (Servicebio,GB111401, 1:500 dilution, China) overnight at 4°C. After being washed with PBS, the sections were incubated with Alexa Fluor 488 (Servicebio, GB25303, 1:500 dilution, China) and Coralite594 (Proteintech, SA00013-3-100, 1:300 dilution, China). Finally, images were captured with an inverted fluorescence microscope (Leica Microsystems, Wetzlar, Germany). Quantification of the positive area was performed using Image Pro Plus 6.0 software. The standard procedure was the same as that used in our previous study (Mao et al., 2023 (link)).
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