Imipramine hydrochloride imp

Vehicle (0.5% sodium carboxymethyl cellulose, SCMC) and drugs were daily administrated at week 4 to week 7 (Figure 6) at 8.00 am. On a behavioral testing day, all treatments were conducted 1 h before testing. The mice were divided into 5 groups (n = 12). The 1st group was a non-stress group (0.5% SCMC, 1 mL/kg, p.o.). The 2nd group was a stressed or UCMS group (0.5% SCMC, 10 mL/kg, p.o.). The 3rd group was the UCMS which received imipramine hydrochloride (IMP) 20 mg/kg, i.p. The 4th and 5th groups were UCMS groups which received LE 100 and LE 500 mg/kg, p.o. The ethanol extract of D. alatus leaf extract (LE, 1 g) was freshly suspended in 0.5% SCMC (10 mL) and used as a stock solution. The reference standard drug, imipramine hydrochloride (IMP) (Sigma-Aldrich, St. Louis, MO, USA) was dissolved in 0.9% saline. One day after finishing behavioral test, the mice were decapitated. All tissues were collected immediately and kept at −80 °C throughout the experiment.