The magnetic moment of the proteins was measured using a superconducting quantum interference device (SQUID) magnetometer. Briefly, protein samples, including wild-type clMagR and mutants (in TBS buffer containing 20 mmol/L Tris, 150 mmol/L NaCl, pH 8.0) and blank controls (20 mmol/L Tris, 150 mmol/L NaCl, pH 8.0), were lyophilized using a freeze dryer (Heto PowerDry LL3000, Thermo Scientific, USA). An MPMS-3 magnetometer (Quantum Design, USA) equipped with a SQUID sensor was used for magnetic measurement of the lyophilized samples at different temperatures (5 K and 300 K). The fields applied were between −2 and 2 T. MH curves (magnetization (M) curves measured versus applied fields (H)) of proteins were obtained after subtracted the background from the buffer control.
Mpms3 magnetometer
Manufactured by Quantum Design
Sourced in United States
The MPMS3 magnetometer is a laboratory instrument designed to measure the magnetic properties of materials. It is capable of performing DC and AC magnetic measurements over a wide range of temperatures and applied magnetic fields. The MPMS3 provides accurate and reliable data for researchers and scientists studying the fundamental magnetic characteristics of various materials.
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Lab products found in correlation
Heto powerdry ll3000, by Thermo Fisher Scientific (2 mentions)
Merlin scanning electron microscope, by Zeiss (1 mentions)
Avance 3 400, by Bruker (1 mentions)
Tga dsc 3, by Mettler Toledo (1 mentions)
D8 advance, by Bruker (1 mentions)
Mpms xl magnetometer, by Quantum Design (1 mentions)
Mini protean tetra system, by Bio-Rad (1 mentions)
Cytation 5 cell imaging multi mode reader, by Agilent Technologies (1 mentions)
Zetasizer nano z, by Malvern Panalytical (1 mentions)
Chemidoc xrs system, by Bio-Rad (1 mentions)
10 protocols using mpms3 magnetometer
1
Magnetic Profiling of Protein Samples
2
Magnetization and Transport Characterization
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Magnetisation loops are measured using a Quantum Design MPMS 3 magnetometer. Angular dependent magnetization measurements are performed using the Quantum Design Horizontal Rotator option. X-ray diffraction and reflectivity is performed on a Bruker D8 diffractometer with an additional four-bounce monochromator to isolate Cu K- at a wavelength of 1.5406 Å. Sheet films are patterned into Hall bars of 5 m width using conventional photolithography and Ar ion milling. Resulting devices are measured using 4-point-probe transport to measure the Hall resistance of the films using a combined Keithley 6221-2182A current source and nano-voltmeter.
3
Magnetic Characterization of Powder Sample
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Magnetic properties were determined using a Quantum Design MPMS3 magnetometer. DC mode was adopted for the measurements of susceptibility and magnetization, while VSM mode was selected for hysteresis, zero-field-cooled (ZFC) magnetization and field-cooled (FC) magnetization (ZFC-FC) and magnetization decay measurements. The sample (0.340 mg) was prepared by drop-casting from carbon disulfide solution onto a slice of Al foil (5.413 mg) which is paramagnetic to minimize the background of sample holder. Then, fast evaporation of carbon disulfide afforded black powder. After that, the Al foil was folded into a small cube and stuck on the inner wall of a plastic straw with a tiny amount of N grease (less than 1 mg). The background of Al foil and Pascal correction were considered when the point-by-point correction was carried on the data.
4
Magnetic Characterization of Avian Magnetoreceptors
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Protein samples, including wild-type robin MagR and pigeon MagR (in buffer E containing 20 mmol/L Tris, 150 mmol/L NaCl, 5 mmol/L D-desthiobiotin, pH 8.0) and blank control (buffer E), were lyophilized using a freezer dryer (Heto PowerDry LL3000, Thermo Scientific, USA). Magnetic measurements were performed on lyophilized samples (mass of approximately 5 mg) using a MPMS-3 magnetometer (Quantum Design, USA) equipped with a superconducting quantum interference device (SQUID) sensor at different temperatures (300 and 5 K). The fields applied were between −2 and 2 T. MH curves (magnetization (M) curves measured versus applied fields (H)) of proteins were obtained after subtracting the background from the buffer control. The volume magnetic susceptibility versus magnetic field of robin and pigeon MagRs were calculated based on the collected data, with a protein density of about 0.15 g/cm3 (Beech et al., 2015 (link)).
5
Comprehensive Materials Characterization
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The EDS results were collected using the Zeiss MERLIN scanning electron microscope (SEM). The atomic resolution HAADF‐STEM images and EELS results were obtained using FEI Titan Themes Cubed G2 300 (Cs Probe) TEM at 300 kV. The nanoflakes' thicknesses were determined by atomic force microscopy (AFM) measurements (Dimension Icon SPM). The magnetization measurements were carried out using the superconducting quantum interference device (SQUID) MPMS3 magnetometer (Quantum Design).
6
Characterization of Organic Ligands
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The 1H nuclear magnetic
resonance (1H NMR) spectra of the organic ligands were
recorded on a Bruker AVANCE III-400 instrument (400 MHz) using dimethyl
sulfoxide-d6 (DMSO-d6) as the NMR solvent. Thermogravimetric analysis (TGA) and
differential scanning calorimetry (DSC) were performed from RT to
600 °C at a heating rate of 10 °C/min under a nitrogen atmosphere
using a Q600 simultaneous DSC-DTA-TGA system from TA Instruments.
Powder X-ray diffraction (PXRD) was performed using synchrotron radiation
(wavelength 1.1 Å) in focused beam configuration in the 2θ
range of 2–30° at 298 K. The simulated PXRD patterns were
calculated from the single-crystal X-ray diffraction (SC-XRD) data
using the Mercury 3.8 program. X-ray photoelectron spectroscopy (XPS)
measurement was performed on an R3000 spectrometer (VG SCIENTA, United
Kingdom) with monochromated Al Kα X-ray radiation as the X-ray
source for excitation. Magnetic measurements of compounds were performed
using a Quantum Design MPMS3 magnetometer for temperatures 3 K ≤ T ≤ 300 K with a 1000 Oe applied field.
resonance (1H NMR) spectra of the organic ligands were
recorded on a Bruker AVANCE III-400 instrument (400 MHz) using dimethyl
sulfoxide-d6 (DMSO-d6) as the NMR solvent. Thermogravimetric analysis (TGA) and
differential scanning calorimetry (DSC) were performed from RT to
600 °C at a heating rate of 10 °C/min under a nitrogen atmosphere
using a Q600 simultaneous DSC-DTA-TGA system from TA Instruments.
Powder X-ray diffraction (PXRD) was performed using synchrotron radiation
(wavelength 1.1 Å) in focused beam configuration in the 2θ
range of 2–30° at 298 K. The simulated PXRD patterns were
calculated from the single-crystal X-ray diffraction (SC-XRD) data
using the Mercury 3.8 program. X-ray photoelectron spectroscopy (XPS)
measurement was performed on an R3000 spectrometer (VG SCIENTA, United
Kingdom) with monochromated Al Kα X-ray radiation as the X-ray
source for excitation. Magnetic measurements of compounds were performed
using a Quantum Design MPMS3 magnetometer for temperatures 3 K ≤ T ≤ 300 K with a 1000 Oe applied field.
7
Synthesis and Characterization of Novel Materials
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All chemical reagents were commercially purchased from Sinopharm Chemical Reagent Co., Ltd. and used without further purification. X-ray powder diffraction data were collected on a Bruker D8 Advance diffractometer with Cu Kα (λ = 1.54056 Å) radiation. Infrared spectra were recorded on a Spectrum Two FT-IR spectrometer using KBr pellets in the range of 4,000–400 cm−1. Thermogravimetric analyses were carried out under N2 atmosphere on a Mettler Toledo TGA/DSC3 synchronous thermal analyzer (heating rate: 10°C·min−1). The vacuum/open 1,200°C tubular electric furnace as well as the ceramic reaction vessel are produced by Tianjin Zhonghuan Co., Ltd. Photoluminescence properties were performed at room temperature using a FLS980 fluorescence spectrophotometer. Magnetic measurements were carried out on a Quantum Design MPMS3 magnetometer in the temperature range of 310–1.8 K.
8
Comprehensive Characterization of Compounds
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Thermogravimetric analysis (TGA) was conducted from RT to 600°C at a heating rate of 10°C min−1 under a nitrogen atmosphere using a TA instrument STD Q600 analyser. Powder X-ray diffraction (PXRD) was conducted using synchrotron radiation (wavelength: 1.1 Å) in a focused beam configuration in the 2θ range 2–20° at 298 K. The simulated PXRD patterns were obtained from single-crystal X-ray diffraction (SCXRD) data using the Mercury software (version 3.8; Macrae et al., 2020 ▸ ). X-ray photoelectron spectroscopy (XPS) measurements for 1 and 2 were performed on an R3000 spectrometer (VG SCIENTA, UK), and XPS measurements for 3 and 4 were performed on a K-ALPHA spectrometer (Thermo VG, UK) with monochromatic Al Kα X-ray radiation as the X-ray source. Magnetic measurements of the compounds were conducted using a Quantum Design MPMS3 magnetometer in the temperature range 3 K ≤ T ≤ 300 K under an applied field of 1000 Oe.
9
Comprehensive Characterization of Compounds
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Elemental analyses (C, H, N) were
performed by the University of Ioannina microanalysis service. Variable-temperature,
solid-state direct current (dc) magnetic susceptibility data were
collected on a Quantum Design MPMS-XL magnetometer at the University
of Zaragoza and a Quantum Design MPMS3 magnetometer at the University
of Glasgow. Diamagnetic corrections were applied to the observed paramagnetic
susceptibilities using Pascal’s constants. Powder X-ray diffraction
(PXRD) measurements were collected on freshly prepared samples of
the complexes on a PANanalytical X’Pert Pro MPD diffractometer
at the University of Crete.
performed by the University of Ioannina microanalysis service. Variable-temperature,
solid-state direct current (dc) magnetic susceptibility data were
collected on a Quantum Design MPMS-XL magnetometer at the University
of Zaragoza and a Quantum Design MPMS3 magnetometer at the University
of Glasgow. Diamagnetic corrections were applied to the observed paramagnetic
susceptibilities using Pascal’s constants. Powder X-ray diffraction
(PXRD) measurements were collected on freshly prepared samples of
the complexes on a PANanalytical X’Pert Pro MPD diffractometer
at the University of Crete.
10
Nanoparticle Characterization Techniques
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High-resolution JEOL transmission electron
microscope (TEM) was used to measure and record the images of nanoparticles.
The hydrodynamic size and ζ potential of the nanoparticles were
measured using a Malvern Zetasizer Nano-ZS (Malvern Panalytical).
The centrifugation was performed on a 5424 Eppendorf and refrigerated
RC 6+ Sorvall centrifuge (Thermo Fisher Scientific). The pH was measured
using a Seven Compact Mettler Toledo pH meter equipped with an InLab
Micro electrode. We performed fluorescence imaging, fluorescence,
and UV–vis absorption measurements using a Cytation 5 cell
imaging multi-mode reader (BioTek Instruments Inc.). Gel electrophoresis
was performed on a Bio-Rad Mini-PROTEAN Tetra system, and blots were
transferred using a Genscript e-blot transfer system. We performed
Western blot imaging and acquisition using Image Lab version 5.2.1
software on a ChemiDoc XRS system from Bio-Rad. Solvent extraction
was performed on a Büchi rotavapor R-124 attached to a water
bath and water-cooled distillation column. Finally, SQUID measurements
were performed on a Quantum Design MPMS 3 magnetometer (Quantum Design).
microscope (TEM) was used to measure and record the images of nanoparticles.
The hydrodynamic size and ζ potential of the nanoparticles were
measured using a Malvern Zetasizer Nano-ZS (Malvern Panalytical).
The centrifugation was performed on a 5424 Eppendorf and refrigerated
RC 6+ Sorvall centrifuge (Thermo Fisher Scientific). The pH was measured
using a Seven Compact Mettler Toledo pH meter equipped with an InLab
Micro electrode. We performed fluorescence imaging, fluorescence,
and UV–vis absorption measurements using a Cytation 5 cell
imaging multi-mode reader (BioTek Instruments Inc.). Gel electrophoresis
was performed on a Bio-Rad Mini-PROTEAN Tetra system, and blots were
transferred using a Genscript e-blot transfer system. We performed
Western blot imaging and acquisition using Image Lab version 5.2.1
software on a ChemiDoc XRS system from Bio-Rad. Solvent extraction
was performed on a Büchi rotavapor R-124 attached to a water
bath and water-cooled distillation column. Finally, SQUID measurements
were performed on a Quantum Design MPMS 3 magnetometer (Quantum Design).
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