For MMP-9 evaluation, a non-stimulated tear fluid sample was collected atraumatically from the lower tear meniscus using a glass micropipette. Typically, 1–3 μl of tears were obtained. Each sample was then placed in a 0.5 ml Eppendorf tube and stored at −20°C until used. The level of MMP-9 was measured using a commercial Human MMP-9 ELISA kit (ThermoFisher Scientific, Human MMP-9 Platinum ELISA). The assay was performed according to the instructions of the manufacturer.
Human mmp 9 platinum elisa
Manufactured by Thermo Fisher Scientific
Sourced in France
The Human MMP-9 Platinum ELISA is a quantitative sandwich enzyme-linked immunosorbent assay (ELISA) designed for the measurement of human Matrix Metalloproteinase-9 (MMP-9) in biological samples.
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Lab products found in correlation
2 protocols using human mmp 9 platinum elisa
1
Quantifying Tear MMP-9 Levels
2
Cytokine and MMP-9 Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cytokine concentrations were determined with the FlowCytomix technology and levels of MMP-9 in cell culture supernatants were measured with the human MMP-9 platinum ELISA (both from Thermofisher, Villebon sur Yvette, France).
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