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Deuterium formaldehyde

Manufactured by Merck Group

Deuterium formaldehyde is a chemical compound with the molecular formula CD2O. It is a stable isotope of formaldehyde, where the hydrogen atoms have been replaced with deuterium atoms. Deuterium formaldehyde is used as a labeling agent in various chemical and biological experiments, allowing researchers to track the movement and incorporation of the deuterium atoms into different molecules or biological systems.

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2 protocols using deuterium formaldehyde

1

Mass Spectrometry Reagent Preparation

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Methanol (MeOH), acetonitrile (ACN), formic acid (FA), ammonium bicarbonate and glacial acetic acid were purchased from Fisher Scientific (Pittsburgh, PA). Borane pyridine, formaldehyde, and deuterium formaldehyde were from Sigma-Aldrich (St. Louis, MO). 2,5-dihydroxybenzoic acid (DHB) was obtained from Acros Organics (Morris Plains, NJ), and alpha-cyano-4-hydroxycinnamic acid (CHCA) was purchased from Sigma-Aldrich (St. Louis, MO). Acidified methanol was prepared as 90% methanol, 9% water and 1% glacial acetic acid (v/v/v). All water used in this study was doubly distilled on a Millipore filtration system (Burlington, MA) or Fisher HPLC grade.
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2

MALDI-TOF Mass Spectrometry Protocol

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Methanol, acetonitrile, formic
acid, acetic acid, and EDTA disodium salt were purchased from Fisher
Scientific (Pittsburgh, PA). Borane pyridine, formaldehyde, and deuterium
formaldehyde were from Sigma-Aldrich (St. Louis, MO). 2,5-Dihydroxybenzoic
acid (DHB) used as MALDI matrix was obtained from MP Biomedicals,
Inc. (Solon, OH). Acidified Methanol was prepared using 90% Methanol,
9% acetic acid, and 1% water.
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