H1975

Manufactured by Procell

Sourced in China

The H1975 is a laboratory equipment designed for general scientific applications. It serves as a versatile tool for various research and testing purposes. The core function of the H1975 is to provide a controlled environment for conducting experiments and analyses.

Automatically generated - may contain errors

Lab products found in correlation

Beas 2b, by Procell (6 mentions) H1299, by Procell (6 mentions) Hcc827, by Procell (4 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (3 mentions) Dulbecco modified eagle medium (dmem), by Nissui Pharmaceutical (1 mentions) Mda mb 231, by Procell (1 mentions) Rpmi 1640, by Thermo Fisher Scientific (1 mentions) Lipofectamine 3000, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin, by Sartorius (1 mentions) Fetal bovine serum (fbs), by Sartorius (1 mentions) H1650, by Procell (1 mentions) Calu 3, by Procell (1 mentions) Penicillin streptomycin, by Beyotime (1 mentions) Hek293t, by Procell (1 mentions) Beas 2b, by Shanghai Zhong Qiao Xin Zhou Biotechnology (1 mentions) Rpmi 1640, by Procell (1 mentions) Dulbecco modified eagle medium (dmem), by Procell (1 mentions) Agomir nc, by GenePharma (1 mentions) Hcc2935, by American Type Culture Collection (1 mentions)

Spelling variants of this product

NCI-H1975 (9 citations)

12 protocols using h1975

LUAD Tissue Collection and Cell Culture

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Sixty paired LUAD tissues and adjacent normal tissues were collected from patients who underwent surgical resection at Taihe Hospital, Hubei University of Medicine. Samples were immediately frozen in liquid nitrogen and stored at –80°C. This study was approved by the Ethics Committee of the Hubei University of Medicine. Informed consent was obtained from all subjects.
LUAD cell lines (A549, H1299, H1975, and H441) and a normal lung epithelial cell line (BEAS-2B) were purchased from the Procell (Wuhan, China). All cells were cultured in DMEM (Gbico, Detroit, MI, United States) supplemented with 10% fetal bovine serum (Hyclone, Logan, UT, United States). Cells were maintained at 37°C, 5% CO₂.

Dong X., Liu Y., Deng X., Shao J., Tian S., Chen S., Huang R., Lin Z., Chen C, & Shen L. (2021). C1GALT1, Negatively Regulated by miR-181d-5p, Promotes Tumor Progression via Upregulating RAC1 in Lung Adenocarcinoma. Frontiers in Cell and Developmental Biology, 9, 707970.

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Immortalized Bronchial and NSCLC Cell Lines

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The immortalized bronchial epithelial cell line (BEAS-2B) and 4 NSCLC cell lines (H520, A549, H1299, and H1975) were all purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China). Cells were maintained in medium containing 10% fetal bovine serum (FBS) (10091-148, GIBCO; Shanghai, China) and 1% penicillin-streptomycin solution, and placed in a cell incubator at 37 °C and 5% CO₂.

Qiao H., Feng Y, & Tang H. (2021). COL6A6 inhibits the proliferation and metastasis of non-small cell lung cancer through the JAK signalling pathway. Translational Cancer Research, 10(10), 4514-4522.

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Cell Viability Assay with Compounds

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H1975, MDA-MB-231, A549, and H1299 cancer cell lines were purchased from the Procell Life Science & Technology Co., Ltd. Cells were cultured at 37 °C in a 5% CO₂ humidified incubator and maintained in high glucose Dulbecco’s Modified Eagle Medium (DMEM, Nissui, Tokyo, Japan) containing 100 mg/mL streptomycin, 2.5 mg/L amphotericin B and 10% heat-inactivated fetal bovine serum (FBS). The cells were inoculated in 96-well culture plates for 12 h and then treated with different concentrations of compounds for 72 h. Water-soluble tezole (WST) reagent was added to each (10 µL) well and cultured at 37 °C for 2 h to assess cell viability. The absorbance was read with a microplate reader at 450 nm. Adriamycin (DOX) was the positive control.

Song Y.T., Yu D.D., Su M.Z., Luo H., Cao J.G., Liang L.F., Yang F, & Guo Y.W. (2023). Structurally Diverse Diterpenes from the South China Sea Soft Coral Sarcophyton trocheliophorum. Marine Drugs, 21(2), 69.

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Characterization of Human Lung Cell Lines

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Human bronchial epithelium cell line (BEAS-2B) and human lung adenocarcinoma cell lines (A549, H1975 and PC9) were purchased from Procell (Procell Life Science&Technology Co., Ltd.). All cells were cultured under standard conditions (37°C, 5% CO₂). All cell lines were authenticated by the short tandem repeat DNA profiling test and checked for absence of mycoplasma contamination.

Shi L., Zou H, & Yi J. (2024). Construction of shared gene signature between rheumatoid arthritis and lung adenocarcinoma helps to predict the prognosis and tumor microenvironment of the LUAD patients. Frontiers in Molecular Biosciences, 10, 1314753.

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NSCLC Cell Line Culture Protocol

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The cell lines used in our study, including human NSCLC cell lines (95-D, H1299, H460, HCC-827, A549, PC-9, and H1975) and human normal bronchial epithelioid cells (HBE) (Procell Life Science and Technology, Wuhan, China), were cultured in Roswell Park Memorial Institute 1640 (RPMI-1640) medium containing 10% fetal bovine serum (Biological, Kibbutz Beit Haemek, Israel) with penicillin and streptomycin and incubated in an environment with 5% CO₂ at 37°C.

Fang Z., Zhong M., Zhou L., Le Y., Wang H, & Fang Z. (2022). Low-density lipoprotein receptor-related protein 8 facilitates the proliferation and invasion of non-small cell lung cancer cells by regulating the Wnt/β-catenin signaling pathway. Bioengineered, 13(3), 6807-6818.

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Lung Cancer Cell Line Manipulation

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The lung cancer cell lines H1975 and A549 were purchased from Procell. The cells are cultured in RPMI1640 (Gibco) supplemented with 10% FBS (Gibco) under conditions of 5% CO2 and 37 °C. siRNA negative control and siALG3 were chemically synthesized by RiboBio (Guangzhou, China). We transfected the siRNA negative and siAGL3 following Lipofectamine 3000 (Invitrogen). After a transfection period of 48 h, cells were collected for subsequent experiments.

Yuan Y., Xie B., Guo D., Liu C., Jiang G., Lai G., Zhang Y., Hu X., Wu Z., Zheng R, & Huang L. (2023). Identification of ALG3 as a potential prognostic biomarker in lung adenocarcinoma. Heliyon, 9(7), e18065.

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Cell Culture Conditions for LUAD

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Six types of human LUAD cell lines (PC9, H358, A549, HCC827, H1299, and H1975) and pulmonary epithelial cell line (BEAS-2B) were provided by the Procell Life Science &Technology Co., Ltd. (Wuhan, China). These cells were maintained in DMEM (Gibco) or RPMI-1640 (Hyclone) supplemented with 10% fetal bovine serum (FBS), 1% streptomycin, and 1% penicillin at 37°C in a humidified atmosphere containing 5% CO₂.

Zou K., Gao P., Xu X., Zhang W, & Zeng Z. (2023). Upregulation of NDUFAF2 in Lung Adenocarcinoma Is a Novel Independent Prognostic Biomarker. Computational and Mathematical Methods in Medicine, 2023, 2912968.

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NSCLC Cell Culture Conditions

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MRC‐5 human embryonic lung fibroblasts, MRC‐5 culture medium, and the NSCLC cell lines H1299, H1975, A549, H1650, and PC9 were obtained from Procell Life Science and Technology. A549 and PC9 cells were cultured in high‐glucose Dulbecco's modified Eagle's medium (Hyclone) containing 10% fetal bovine serum (FBS; Biological Industries) and 1% penicillin/streptomycin (Biological Industries), whereas other NSCLC cell lines were grown in RPMI‐1640 medium (Hyclone) supplemented with 10% FBS and 1% penicillin/streptomycin. All cells were incubated in a humidified incubator with 5% CO₂ at 37°C.

Chen R., Wang Z., Lu T., Liu Y., Ji Y., Yu Y., Tou F, & Guo S. (2023). Budding uninhibited by benzimidazoles 1 overexpression is associated with poor prognosis and malignant phenotype: A promising therapeutic target for lung adenocarcinoma. Thoracic Cancer, 14(10), 893-912.

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LUAD Patient-Derived Tissue and Cell Line Protocol

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All tumor and peritumor tissues were surgically resected from LUAD patients at the Second Hospital of Shandong University (Jinan, China). Each patient had an independent pathological review-confirmed diagnosis of LUAD. All studies were performed under supervision and approved by the Ethics Committee of the Second Hospital of Shandong University (KYLL-2021(LW)085), and adhered to the Declaration of Helsinki. Informed consent was obtained from human subjects. The privacy rights of human subjects must always be observed.
The LUAD cell lines, including A549, H1299, PC9 and H1975, were purchased from Procell Life Science & Technology Co. Ltd. (Wuhan, China). All LUAD cells were cultured in complete medium (RPMI 1640 for A549, H1299, H1975 or DMEM for PC9, plus 10% FBS). Cells were cultured in a 37 °C incubator with a humidified 5% CO2 atmosphere.

Wu Q., Li R., Wang Q.X., Zhang M.Y., Liu T.T, & Qu Y.Q. (2022). Junctional adhesion molecule-like protein promotes tumor progression via the Wnt/β-catenin signaling pathway in lung adenocarcinoma. Journal of Translational Medicine, 20, 260.

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Cell Line Cultivation and Characterization

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The human embryonic kidney (HEK) 293T, human immortalized bronchial epithelial BEAS-2B, and NSCLC H1299, A549, LTEP-α-2, H226, Calu-3, HCC827, and H1975 cell lines were purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China). The cells were cultured in DMEM high-glucose medium or RPMI-1640 medium (HyClone, South Logan, UT, USA) containing 10% fetal bovine serum (FBS; Gibco, Carlsbad, CA, USA) and 1% penicillin-streptomycin (Beyotime, Shanghai, China) in a humidified atmosphere with 5.0% CO₂ at 37 °C. The cell supplier had confirmed the genetic characteristics of these cells. All these cell lines were passaged within 6 months.

Zeng Y., Zhao J., Wu Z., Huang Y., Wang A., Zhu J., Xu M., Zhang W., Zhang X., Li J., Huang J.A, & Liu Z. (2024). Targeting TYK2 alleviates Rab27A-induced malignant progression of non-small cell lung cancer via disrupting IFNα-TYK2-STAT-HSPA5 axis. NPJ Precision Oncology, 8, 74.

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