Ab1954

Select strains were grown overnight in YPD and cell wall susceptibility assays were performed as described elsewhere (Ram & Klis, 2006). Congo red and Calcofluor white dyes (cat. nos. C6277 and F3543; Millipore Sigma) were added to final concentrations of 150 and 20 µg/mL, respectively.
Quantitative polymerase chain reaction (PCR) was performed according to PowerUp SYBR Green kit instructions (Thermo Fisher Scientific) using a Roche LightCycler 480. β‐Actin, rhGH, rhG‐CSF, and trastuzumab ORFs were PCR amplified and serially diluted to generate a standard curve (see Table S2 for primers). Experimental design and absolute copy number quantification were performed according to best practices (Abad et al., 2010)
Protein concentrations of rhGH and rhG‐CSF were determined using sandwich enzyme‐linked immunosorbent assay (ELISA) as described elsewhere (Crowell et al., 2018). rhGH‐specific antibodies were used at concentrations of 5 µg/ml capture (ab1954; Abcam, Cambridge, UK) and 0.6 µg/ml secondary/detection (ab1956; Abcam). rhG‐CSF‐specific antibodies were used at concentrations of 2 µg/ml capture (BVD13‐3A5; Biolegend, San Diego, CA), 0.4 µg/ml secondary (BVD11–37G10), and 0.2 µg/ml detection (ab7403; Abcam). Protein concentrations of trastuzumab were determined using a Human IgG1 ELISA kit (cat. no. RAB0242; Millipore Sigma).