Nu7026

Hypoosmotic stress was applied by incubation the cells in 50% DMEM/50% H2O for 30 min–3 h. Hyperosmotic stress was applied by incubation in DMEM supplemented with 600 mM NaCl for 1 h. For kinase inhibition experiments, cells were treated with 20 μM KU55933 (Tocris Bioscience) for 3 h, 15 μM VE821 (Sigma) for 3 h or 50 μM NU7026 (Adooq Bioscience) for 6 h. For transcription inhibition experiments, cells were treated with 0.01 μg/ml actinomycin D (Biotium) for 3 h, or 50 μM DRB (Santa Cruz Biotechnology) for 3 h. For induction of DSBs, the cells were treated with 1–20 μg/ml etoposide (Sigma) for 1 h. I-PpoI nuclear translocation was initiated by incubation the cells with 5 μM 4-hydroxytamoxifen (4-OHT) (Sigma) for 4–16 h.

Z-VAD-FMK, chloroquine (CQ), Rapamycin (Rapa), NU7441, Cyt387, A674563, KU60019, LY294002, PD0332991, AT7519, MK2206, CUDC907, LY2109761, GANT61, BIX 02189, Spautin-1, QNZ, LY317615, PD169316, GSK2606414, LYK974, TCK ERK 11e, SC79, MC1568, H89, ICG001, Perifosine, AEE788, ABT263, GDC-0941, FH535, PD0325025, NU7026, STF-083010, AEBSF HCl were purchased from Adooq. N-Acetyl-l-cysteine ethyl ester, DMSO, PEG300, and Tween-80 were purchased from MCE.