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Anti col6a1

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Anti-COL6A1 is a primary antibody that targets the COL6A1 protein, a key component of type VI collagen. It is designed for use in various applications, such as immunohistochemistry and western blotting, to detect and analyze the presence and distribution of COL6A1 in biological samples.

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Lab products found in correlation

Anti ngf, by Abcam (1 mentions) Anti wnt3a, by Santa Cruz Biotechnology (1 mentions) Anti fgf18, by Santa Cruz Biotechnology (1 mentions) Facscanto 2 cytometer, by FlowJo (1 mentions) Fixation and permeabilization solution, by BD (1 mentions) Anti hsp90, by Santa Cruz Biotechnology (1 mentions) Novex ecl chemiluminescent substrate reagent kit, by Thermo Fisher Scientific (1 mentions) Lumigen, by GE Healthcare (1 mentions) Anti cd81, by Santa Cruz Biotechnology (1 mentions) Anti cd9, by Santa Cruz Biotechnology (1 mentions) Anti hsp70, by Santa Cruz Biotechnology (1 mentions) Nitrocellulose membrane, by Bio-Rad (1 mentions)

3 protocols using anti col6a1

1

Detailed Immunostaining Protocol for Tissue Analysis

Cited 1 time
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Immunostaining was performed as described previously (Liu et al., 2019b (link)). After antigen retrieval and blocking with serum for 1 h, the slices were incubated with the primary antibodies at 4°C overnight; these antibodies included anti-inhba (Santa Cruz, sc-166503), anti-wnt3a (Santa Cruz, sc-74537), anti-col6a1 (Santa Cruz, sc-377143), anti-wnt16 (Santa Cruz, sc-271897), anti-fgf18 (Santa Cruz, sc-393471), anti-chrm1 (Santa Cruz, sc-365966), anti-ngf (Abcam, ab52987), and anti-gdf7 (Abcam, ab189928). After washing three times with PBS, the slices were subjected to related secondary antibodies for 1 hour at room temperature. They were then washed three times with PBS (5 min each time), followed by incubation with diamidino-phenyl-indole (DAPI) for 30 min. Images were taken by laser scanning confocal fluorescence microscopy (Leica SP8).
Cai S., Si N., Wang Y, & Yin N. (2023). Transcriptomic analysis of the upper lip and primary palate development in mice. Frontiers in Genetics, 13, 1039850.
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2

Synovial Cell Immunophenotyping

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Synovial cells were fixed with a Fixation and Permeabilization Solution (BD Biosciences, cat: #554722). Cells were then incubated with anti-VEGF-C (NOVUS NB110-61022) and anti-COL6A1 (Collagen type VI alpha 1 chain) antibody (Santa Cruz 377143), followed by APC-anti-rabbit IgG and PE-anti-mouse IgG antibody. Cells were subjected to flow cytometric analysis using a Becton-Dickinson FACSCanto II Cytometer, and analyzed with Flowjo10.4 software (FLOWJO, LLC Ashland).
Gelfand M.S., Mironov A.A, & Pevzner P.A. (1996). Gene recognition via spliced sequence alignment.. Proceedings of the National Academy of Sciences of the United States of America, 93(17), 9061-9066.
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3

Western Blot Analysis of Protein Targets

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Fifty µg protein from each cell lysate were subjected to reducing SDS-polyacrylamide gel electrophoresis (SDS-PAGE) using 12 % resolving gels for 10-70 kDa proteins of interest or 10 % resolving gels for 20-100 kDa proteins of interest. SDS-PAGE was performed at 120 V at room temperature. Resolved proteins were subsequently transferred to nitrocellulose membranes (Bio-Rad, USA) at 80 V for 2 h at room temperature. The blot was blocked with 5 % (w/v) skimmed milk in 0.05% (v/v) Tween 20/Tris-buffered saline for 1h at room temperature, and probed with respective antibodies, followed by secondary antibodies dissolved in 5 % (w/v) skimmed milk. The blots were developed with either Novex ® ECL Chemiluminescent Substrate
Reagent Kit (Invitrogen) or Lumigen ® (GE Healthcare). All western blot analyses were conducted with at least three independent experiments. The antibodies used in our work were anti-POPX2 (self-raised), anti-CD81 (Santa Cruz), anti-CD9 (Santa Cruz), anti-HSP70 (Santa Cruz), anti-HSP90 (Santa Cruz), anti-COL6A1 (Santa Cruz), anti-LAMA5 (Santa Cruz), anti-
Zhang S., Weng T., Cheruba E., Guo T., Chan H., Sze S.K, & Koh C.G. (2017). Phosphatase POPX2 Exhibits Dual Regulatory Functions in Cancer Metastasis. Journal of proteome research, 16(2).
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