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Anti p70 s6 kinase

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Anti-p70 S6 Kinase is a laboratory reagent used for the detection and quantification of p70 S6 kinase, a serine/threonine-protein kinase that plays a key role in the regulation of cell growth, proliferation, and protein synthesis. This antibody can be used in various immunological techniques, such as Western blotting, to measure the expression levels of p70 S6 kinase in biological samples.

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Lab products found in correlation

Anti phospho ampkα thr172, by Cell Signaling Technology (2 mentions) Anti β actin, by Cell Signaling Technology (2 mentions) Anti phospho p70 s6 kinase thr389, by Cell Signaling Technology (2 mentions) Anti ampkβ 1 2, by Thermo Fisher Scientific (2 mentions) Anti phospho s6 ribosomal protein ser235 236, by Santa Cruz Biotechnology (2 mentions) Anti s6 ribosomal protein, by Santa Cruz Biotechnology (2 mentions) Anti nmt1, by Abcam (2 mentions) Anti mtor 7c10, by Cell Signaling Technology (2 mentions) Cd133, by Abcam (1 mentions) Anti β actin peroxidase, by Merck Group (1 mentions) Anti s6 ribosomal protein, by Cell Signaling Technology (1 mentions) N acetylcysteine, by Merck Group (1 mentions) Anti n cadherin, by Cell Signaling Technology (1 mentions) Anti e cadherin, by Cell Signaling Technology (1 mentions) Anti tsc2, by Cell Signaling Technology (1 mentions) Torin 1, by Cayman Chemical (1 mentions) Anti phospho s6 ribosomal protein, by Cell Signaling Technology (1 mentions) Anti sestrin2, by Proteintech (1 mentions) Anti β actin, by Developmental Studies Hybridoma Bank (1 mentions) Rapamycin, by LC Laboratories (1 mentions)

Close spelling variants of this product

P70 S6 Kinase (7 citations) Anti-S6 (3 citations)

5 protocols using anti p70 s6 kinase

1

Immunoblot Analysis of Protein Expression

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Whole-cell lysates were homogenized in modified RIPA buffer14 (link) and assayed by immunoblot as previously described14 (link). The primary antibodies against FKBP51 (rabbit polyclonal; Novus Biological), FKBP51s13 (link) and CD274/PD-L1 (rabbit polyclonal; Novus Biological) were used diluted 1:2500. CD133 (rabbit polyclonal; Abcam; Cambridge, UK) was used diluted 1:1000. A further antibody Pdcd-1L1 (rabbit polyclonal, Santa Cruz Biotechnology; Santa Cruz, CA, USA) was used for PD-L1 detection at the 1:1000 dilution. Antibody against M2-Flag, caspase-7 and γ-Tubulin (mouse monoclonal; Sigma-Aldrich, St. Louis, MO, USA) were used diluted 1:5000. Anti β-Actin-Peroxidase (mouse monoclonal; Sigma-Adrich) was used diluted 1:10000. Anti-phospho-Akt (Ser473), Akt1/2/3, phosphor-S6 kinase, G3PDH and Sox-2 (rabbit monoclonal; Cell Signaling, Danvers, MA, USA) were used diluted 1:1000. Anti-p70S6 kinase (rabbit polyclonal; Santa Cruz) was used diluted 1:500.
D’Arrigo P., Digregorio M., Romano S., Tufano M., Rea A., Hausch F., Dedobbeleer M., Vigorito V., Russo S., Bauder M., Rogister B, & Romano M.F. (2019). The splicing FK506-binding protein-51 isoform plays a role in glioblastoma resistance through programmed cell death ligand-1 expression regulation. Cell Death Discovery, 5, 137.
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2

Immunoblotting Experimental Conditions

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Experimental conditions for immunoblotting experiments have been previously published5 (link), 7 . Primary antibodies used were as follows: anti-NMT1 (Abcam, ab84666), anti-phospho-AMPKα (Thr172) (Cell Signaling Technology, 40H9), anti-AMPKα−1, 2 (Abcam, 34.2), anti-AMPKβ−1,2 (Thermo Fisher Scientific, E.427.6), anti-AMPKγ−1 (Labome, Y308), anti-mTOR (7C10) (Cell Signaling Technology, 2983S), anti-phospho-p70 S6 Kinase (Thr389) (Cell Signaling Technology, 9205S), anti-p70 S6 Kinase (Santa Cruz Biotechnology, B-5), anti-phospho-S6 ribosomal protein (Ser235/236) (Santa Cruz Biotechnology, 50.Ser 235/236) and anti-S6 ribosomal protein (Santa Cruz Biotechnology, C-8). β-actin expression detected with anti-β-actin (Cell Signaling Technology, 8H10D10) served as the internal control.
Wen Z., Jin K., Shen Y., Yang Z., Li Y., Wu B., Tian L., Shoor S., Roche N.E., Goronzy J.J, & Weyand C.M. (2019). N-myristoyltransferase deficiency impairs AMPK activation and promotes synovial tissue inflammation. Nature immunology, 20(3), 313-325.
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3

Immunoblotting of Sestrin2 and mTOR Pathway

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Antibodies used for immunoblotting included anti-sestrin2 obtained from Proteintech Group, anti-phospho-p70 S6 Kinase, anti-phospho-S6 Ribosomal Protein, anti-S6 Ribosomal Protein, anti-E-cadherin, anti-N-cadherin, and anti-TSC2 from Cell Signaling Technology, anti-p70 S6 Kinase from Santa Cruz Biotechnology, anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) from Aviva Systems Biology, and anti-β-Actin from Developmental Studies Hybridoma Bank. Rapamycin was obtained from LC Laboratories. Torin 1 was purchased from Cayman Chemical. N-acetyl cysteine (NAC) was purchased from Sigma-Aldrich.
Shin J., Bae J., Park S., Kang H.G., Shin S.M., Won G., Kim J.S., Cho S.G., Choi Y., Oh S.M., Shin J., Kim J.S, & Park H.W. (2020). mTOR-Dependent Role of Sestrin2 in Regulating Tumor Progression of Human Endometrial Cancer. Cancers, 12(9), 2515.
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4

Immunoblotting Experimental Conditions

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Experimental conditions for immunoblotting experiments have been previously published5 (link), 7 . Primary antibodies used were as follows: anti-NMT1 (Abcam, ab84666), anti-phospho-AMPKα (Thr172) (Cell Signaling Technology, 40H9), anti-AMPKα−1, 2 (Abcam, 34.2), anti-AMPKβ−1,2 (Thermo Fisher Scientific, E.427.6), anti-AMPKγ−1 (Labome, Y308), anti-mTOR (7C10) (Cell Signaling Technology, 2983S), anti-phospho-p70 S6 Kinase (Thr389) (Cell Signaling Technology, 9205S), anti-p70 S6 Kinase (Santa Cruz Biotechnology, B-5), anti-phospho-S6 ribosomal protein (Ser235/236) (Santa Cruz Biotechnology, 50.Ser 235/236) and anti-S6 ribosomal protein (Santa Cruz Biotechnology, C-8). β-actin expression detected with anti-β-actin (Cell Signaling Technology, 8H10D10) served as the internal control.
Wen Z., Jin K., Shen Y., Yang Z., Li Y., Wu B., Tian L., Shoor S., Roche N.E., Goronzy J.J, & Weyand C.M. (2019). N-myristoyltransferase deficiency impairs AMPK activation and promotes synovial tissue inflammation. Nature immunology, 20(3), 313-325.
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5

Phosphorylated Muscle Protein Analysis

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Phosphorylated proteins were prepared from skeletal muscle cryosections with some modifications according to the previous report (Yonekawa et al., 2014 (link)). Proteins were extracted in 1% Triton X-100/Tris buffered saline in the presence of 1 mM Na3VO4, 10 mM NaF and protein inhibitor cocktail (Roche). SDS-PAGE was done following Laemmli's method. Membranes were incubated with the following antibodies: anti-collagen VI (Santa Cruz, 1:250), anti-tubulin (Sigma, 1:250), anti-Akt1 (CST, 1:1000), anti-S473 phosphoAkt (CST, 1:200), anti-p70S6 kinase (Santa Cruz, 1:200), anti-S411 phosphoS6 kinase (Santa Cruz, 1:200), and anti-T421/S424 phosphoS6kinase (Santa Cruz, 1:200). After washing, membranes were incubated in appropriate HRP-labeled secondary antibodies, washed, and then made to react with Immobilon Western Chemiluminescent HRP Substrate. Detection and quantification of band intensities were done by ImageQuant LAS 4000 system (GE healthcare).
Noguchi S., Ogawa M., Malicdan M.C., Nonaka I, & Nishino I. (2016). Muscle Weakness and Fibrosis Due to Cell Autonomous and Non-cell Autonomous Events in Collagen VI Deficient Congenital Muscular Dystrophy. EBioMedicine, 15, 193-202.
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