Biotinylated DNA was generating using a forward primer containing a Biotin TEG group on the 5′ end obtained from Sigma‐Aldrich: Biotin TEG 5′, and a reverse Reverse 3′ primer (see resource table for primer sequences). At least 2 ml of total PCR volume (performed in 50 μl reactions) for each individual promoter sequence was amplified individually for each replicate. PCR reactions were pooled and DNA was purified using AMPURE beads and eluted in water. For each sample, 50 μl of Dyna M280 Streptavidin was used and coupled to 15 μg of cleaned biotinylated PCR product according to the manufacturer's recommendations. The beads were placed in an equivalent volume of DBB (150 mM NaCl, 50 mM Tris/HCl pH, 8.0, 10 mM MgCl2) and used immediately for DNA affinity purification assay.
Biotin teg
Manufactured by Merck Group
Biotin TEG is a laboratory reagent produced by Merck Group. It is a compound containing biotin, a water-soluble vitamin, and a triethylene glycol linker. The core function of Biotin TEG is to serve as a versatile tool for various bioanalytical applications, such as protein labeling and detection.
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Lab products found in correlation
2 protocols using biotin teg
1
Biotinylated DNA Affinity Purification
2
Biotin-labeled DNA Nanostructure Assembly
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To bind the SA, 3′ end of a staple strand (5′-CGA AGC ACT CAT TTT TGG GAA CTG GAG TTA TCC CTA TTT TTT CCT GAA GTA C) at the corner of square 5 of SnL was modified with BiotinTEG and was purchased from Sigma-Aldrich. The monomeric Sn for dimers and 1D chains were assembled with staple strand with biotin and purified according to the method described above. The assembled dimers and 1D chains from the 10 nM monomeric Sn were mixed with SA at a ratio of 1 : 3 and incubated 1 h at room temperature. Then, the excess SA was removed using the Microspin S-400 HR column (GE Healthcare Life Sciences) following the manufacturer's protocol.
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