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Antibody against β actin actb

Manufactured by Merck Group
Sourced in United States

Antibody against β-actin (ACTB) is a laboratory tool used to detect and quantify the presence of the β-actin protein in biological samples. β-actin is a ubiquitous cytoskeletal protein found in all eukaryotic cells and is commonly used as a reference protein in various experimental techniques.

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2 protocols using antibody against β actin actb

1

Antibody-based Protein Expression Analysis

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Antibodies against AnxA6 as well as secondary anti-mouse, anti-goat, and anti-rabbit horseradish peroxidase-conjugated antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). Antibody against HIF-1α was purchased from BD Biosciences (Franklin Lakes, NJ, USA). Antibodies against HIF-2α and EGFR, were purchased from Cell Signaling Technology (Beverly, MA, USA). Antibody against β-actin (ACTB) was purchased from Sigma Aldrich (St. Louis, MO, USA). Glucose uptake assay kit (Fluorometric; ab136956) and ATP Assay Kit (Colorimetric/Fluorometric; ab83355) were purchased from Abcam (Cambridge, UK). A set of EGFR-TKIs including lapatinib-ditosylate, erlotinib, gefitinib, and canertinib were purchased from BioVision Inc. (Milpitas, CA, USA). The AR antagonists bicalutamide and enzalutamide were purchased from Sigma Aldrich (St. Louis, MO, USA). Except otherwise indicated, all other reagents were purchased from Sigma Aldrich and/or Thermo Fisher Scientific (Waltham, MA, USA).
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2

Antibody-based Proteomic Analysis Protocol

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Antibodies against AnxA6, as well as secondary anti-mouse, anti-goat, and anti-rabbit horseradish peroxidase-conjugated antibodies were purchased from Santa Cruz Biotechnology (Dallas, TX, USA). The antibodies against COXIV, Cytc. C, Pyruvate dehydrogenase (PDH), and Superoxide dismustase (SOD1) were purchased from Cell Signaling Technology (Beverly, MA, USA). Oil Red-O, Rotenone, 2-Deoxy-D-Glucose, Etomoxir, and antibody against β-actin (ACTB) were purchased from Sigma Aldrich (St. Louis, MO, USA). A set of EGFR-TKIs including lapatinib-ditosylate, erlotinib, gefitinib, and canertinib were purchased from BioVision (Milpitas, CA, USA). The Mito Stress Test Assay, Palmitate FAO Assay, and the culture media were purchased from Agilent Technologies (Santa Clara, CA, USA). Except where otherwise indicated, all the other reagents were purchased from Sigma Aldrich and/or Cell Signaling Technology.
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