Anti p110

Manufactured by Cell Signaling Technology

Sourced in China, United States

Anti-p110 is a laboratory reagent that recognizes the p110 subunit of the phosphoinositide 3-kinase (PI3K) enzyme. It is used in research applications to detect and study the expression and localization of the p110 protein.

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Lab products found in correlation

4 protocols using anti p110

Western Blot Antibody Panel for Cell Signaling

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Anti-CDC37L1 (16293-1-AP, Proteintech, China), anti-β-actin (sc-81178, Santa Cruz Biotechnology, Dallas, TX, USA), anti-CDK6 (14052-1-AP, Proteintech, China), anti-CDK4 (#12790, Cell Signaling Technology, USA), anti-Cyclin D1 (#2922, Cell Signaling Technology, USA), anti-FAK (66258-1-Ig, Proteintech, China), anti-P110 (#4255, Cell Signaling Technology, USA) and anti-mTOR (#2983, Cell Signaling Technology, USA).

Li L., Tao X., Li Y., Gao Y, & Li Q. (2021). CDC37L1 acts as a suppressor of migration and proliferation in gastric cancer by down-regulating CDK6. Journal of Cancer, 12(11), 3145-3153.

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Antibody Immunostaining Protocol

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All antibodies were purchased as follows: anti-NEDL2 (ab92711, Abcam), anti-Ret (ab134100, Abcam), anti-Neurofilament (ab50284, Abcam), anti-Akt (sc-8312, Sata Cruze), anti-pAkt (#4060, Cell Signaling), anti-S6K1 (#9202, Cell Signaling), anti-pS6K1 (#9234, Cell Signaling), anti-p85 (#4257, Cell Signaling), anti-p110 (#4249, Cell Signaling), anti-BrdU (#5259, Cell Signaling), anti-Myc (MBL), anti-Flag (MBL), anti-Hsp90 (sc-101494, Santa Cruz).

Qiu X., Wei R., Li Y., Zhu Q., Xiong C., Chen Y., Zhang Y., Lu K., He F, & Zhang L. (2016). NEDL2 regulates enteric nervous system and kidney development in its Nedd8 ligase activity-dependent manner. Oncotarget, 7(21), 31440-31453.

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Comprehensive Antibody Validation for Western Blotting and Immunoprecipitation

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Human anti-LZTS2 antibodies were obtained from Proteintech (15677-1-AP) (for western blotting) and Bethyl (A304-938A-T) (for IP). Human anti-β-Trcp (#4394), anti-HA (#3724), anti-phospho-AKT (#4060), anti-AKT (#4685), anti-p85 (#4257), anti-p110 (#3011), anti-CK1ε (#12448), anti-Snail (#3879), anti-Slug (#9585), anti-N-Cadherin (#13116), anti-E-Cadherin (#3195), anti-ZO-1 (#8193), anti-β-Catenin (#8480), anti-Vimentin (#5741), anti-TCF8 (#3396), and anti-Claudin-1 (#13255) antibodies were obtained from Cell Signaling Technology. Human anti-S6K1 (sc-8418), anti-GAPDH (sc-47724) and anti-Myc (sc-40) antibodies were purchased from Santa Cruz Biotechnology. Anti-CK1δ (14388-1-AP), anti-CK2α (10992-1-AP), anti-CDK1 (19532-1-AP), anti-GRK2 (13990-1-AP), anti-VRK2 (12946-1-AP), anti-RSK1 (16463-1-AP), anti-RSK2 (23762-1-AP) and anti-PKCα (21991-1-AP) antibodies were obtained from Proteintech. Anti-Flag (F7425) antibody was purchased from Sigma-Aldrich. Anti-CK1α (ab82426) antibody was purchased from Abcam. Anti-ATM (A19650), anti-α-tubulin (AC012) and anti-Lamin B1 (A11495) antibody were obtained from ABclonal Technology. Anti-phospho-p85 antibody (AF5905) was purchased from Beyotime Biotechnology. Cycloheximide (CHX, A8244) and MG132 (474790) were purchased from Sigma-Aldrich and Millipore, respectively.

Lu Y., Li X., Liu H., Xue J., Zeng Z., Dong X., Zhang T., Wu G., Yang K, & Xu S. (2021). β-Trcp and CK1δ-mediated degradation of LZTS2 activates PI3K/AKT signaling to drive tumorigenesis and metastasis in hepatocellular carcinoma. Oncogene, 40(7), 1269-1283.

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Protein Expression Analysis of ESCC

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Cultured ESCC cells and patient samples were homogenized in RIPA lysis buffer (Beyotime) in the presence of 1 mM PMSF (Beyotime) and lysed on ice for 30 min, followed by centrifugation at 14,000 × g for 10 min at 4°C to harvest the supernatant. Protein concentrations were determined using the BCA Protein Assay Kit (Beyotime) and normalized. Proteins were separated via SDS-PAGE and analyzed using the standard western blotting protocol. GAPDH was used as the internal control. The following primary antibodies were used: anti-BRE (#ab177960, 1:1000, Abcam, UK), anti-AKT (#2920, 1:1000, Cell Signaling Technology, USA), anti-p-AKT (#4060, 1:1000, Cell Signaling Technology), anti-mTOR (#2983, 1:1000, Cell Signaling Technology), anti-p-mTOR (#5536, 1:1000, Cell Signaling Technology), anti-PTEN (#9188, 1:1000, Cell Signaling Technology, USA), anti-p110 (#4249, 1:1000, Cell Signaling Technology), anti-p85 (#4292, 1:1000, Cell Signaling Technology), and anti-GAPDH (#5174, 1:1000, Cell Signaling Technology) antibodies.

Jin F., Zhu Y., Chen J., Wang R., Wang Y., Wu Y., Zhou P., Song X., Ren Z, & Dong J. (2020). BRE Promotes Esophageal Squamous Cell Carcinoma Growth by Activating AKT Signaling. Frontiers in Oncology, 10, 1407.

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