The determination method of ABTS free radicals was modified according to the previous method (Thaipong et al., 2006 (link)). ABTS free radical ion working solution was a mixture of 7.4 mM ABTS (Shanghai McLean Biochemical Technology Co., Ltd., China) stock solution and 2.6 mM potassium persulfate solution and must be placed at room temperature in the dark condition for 12 h. The sample was mixed with the diluted ABTS working solution and reacted at 37°C for 30 min, and an equal volume of 95% ethanol was used as a control group instead of the sample. The absorbance was measured at 734 nm and marked them as As and A0, respectively. Each experiment was conducted in triplicate. The ABTS radical scavenging rate was calculated with the following formula:
2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts)
Manufactured by Maclin Biochemical Technology
Sourced in China
ABTS is a laboratory reagent used for colorimetric detection and quantification. It is a substrate for various enzymes and can be used in various assays, including antioxidant capacity and enzyme activity measurements.
Automatically generated - may contain errors
Lab products found in correlation
2 2 diphenyl 1 picrylhydrazyl (dpph), by Maclin Biochemical Technology (14 mentions)
Total dietary fiber assay kit tdf 200a, by Megazyme (3 mentions)
Gallic acid, by Maclin Biochemical Technology (2 mentions)
Porcine bile salts, by Solarbio (1 mentions)
α amylase, by Yuanye Bio-Technology (1 mentions)
Ethanol, by Shanghai Titan Scientific (1 mentions)
Urea, by Maclin Biochemical Technology (1 mentions)
Trypsin, by Yuanye Bio-Technology (1 mentions)
Hyaluronidase, by Yuanye Bio-Technology (1 mentions)
Hydrochloric acid (hcl), by Chron Chemicals (1 mentions)
Normal human serum, by Solarbio (1 mentions)
D glucose monohydrate, by Chron Chemicals (1 mentions)
Sodium chloride (nacl), by Chron Chemicals (1 mentions)
Vanillin, by Maclin Biochemical Technology (1 mentions)
Glycerol, by Maclin Biochemical Technology (1 mentions)
Quercetin, by Maclin Biochemical Technology (1 mentions)
2 4 6 tripyridyl striazine, by Maclin Biochemical Technology (1 mentions)
Catechin, by Maclin Biochemical Technology (1 mentions)
Rutin hydrate, by Maclin Biochemical Technology (1 mentions)
Folin ciocalteu s phenol reagent, by Maclin Biochemical Technology (1 mentions)
11 protocols using 2 2 azinobis 3 ethylbenzothiazoline 6 sulfonic acid (abts)
1
Antioxidant Activity Determination by ABTS Method
2
Extraction and Characterization of Lotus Leaves
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Dry lotus (Nelumbo) leaves was provided by Hunan Zhenxing Traditional Chinese Medicine Co., Ltd. (Changsha, Hunan, China). Cellulase (CAS 9012-54-8, S10041) was bought from Shanghai Yuanye Biotechnology Co., Ltd (Shanghai, China). Total dietary fiber assay kit (TDF-200A) was bought from Megazyme International Ireland Ltd (Bray, Ireland). Rutin, hyperoside, isoquercitrin, astragalin and quercetin were bought from Chengdu Aifa Biotechnology Co., Ltd (Chengdu, Sichuan, China). Catechin, myricetin and kaempferol were bought from Hefei Bome Biotechnology Co. Ltd (Hefei, Anhui, China). DPPH and ABTS were brought from Shanghai Maclean Biochemical Technology Co. Ltd (Shanghai, China). The rest of reagents were brought from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China) and were analytical pure.
3
Physicochemical and Bioactive Properties
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PO was provided by Luck Huaguagn Graphics Co., Ltd. Sodium starch octenylsuccinate and inulin were purchased from Thousand Taste Food Technology Co., Ltd. (Shanghai, China), and Baiyun Aroma Chemical Co., Ltd. (Guangzhou, China), respectively. Organic reagents such as ethanol (99.7%) and hexane were of analytical grade and purchased from Shanghai Titan Technology Co., Ltd. (Shanghai, China). α-amylase was from Yuanye, Shanghai, China, trypsin, pancreatic lipase, and potassium persulfate from Aladdin, Shanghai, China, porcine bile salts from Solarbio, Beijing, China, and DPPH and ABTS were from Macklin, Shanghai, China.
4
Optimizing Enzyme and Reagent Sourcing
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Ascorbate oxidase, superoxide dismutase (SOD), L-glutamate sodium, trypsin, and hyaluronidase were obtained from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). ABTS, isopropyl alcohol (IPA), K4[Fe(CN)6], and urea were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). Copper(II) chloride dihydrate (CuCl2·2H2O) was obtained from Shanghai Titan Scientific Co., Ltd. (Shanghai, China). L-Serine was purchased from Tianjin Guangfu Fine Chemical Research Institute (General Partnership) (Tianjin, China). L(+)-Ascorbic acid, hydrochloric acid (HCl), D-glucose monohydrate, sodium chloride (NaCl), and potassium chloride (KCl) were purchased from Chengdu Chron Chemicals Co., Ltd. (Chengdu, China). Normal human serum was purchased from Beijing Solarbio Science and Technology Co., Ltd. (Beijing, China). Sodium dihydrogen phosphate (NaH2PO4), K3[Fe(CN)6], and bovine albumin were obtained from Shanghai Adamas Reagent Co., Ltd. (Shanghai, China).
5
Extraction and Characterization of Dried LSP
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The dried LSP discarded during processing were collected from Hangzhou, Zhejiang province, China, and dried with hot air at 45 °C until reaching a constant weight. Folin-Ciocalteu phenol reagent, quercetin, vanillin, and glycerol were purchased from Macklin, gallic acid, DPPH, ABTS, 2,4,6-tripyridyl-s-triazine (TPTZ), ferric chloride, sodium carbonate (Na2CO3), sodium nitrite (NaNO2), aluminum chloride (AlCl3), sodium hydroxide (NaOH), methanol, hydrochloric acid, catechin, Folin-Denis reagent, tannin acid, Trolox, phosphate, potassium ferricyanide, trichloroacetic acid, ferric chloride, and L-ascorbic acid were purchased from Aladdin Industrial Co. (Shanghai, China). All solvents of HPLC grade were purchased from Aladdin Industrial Co., Ltd. (Shanghai, China).
6
Wheat Antioxidant Properties Evaluation
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The wheat seeds were kindly provided by a local farmer in Lingbi County, Suzhou City, Anhui Province, China. Hoagland Nutrition reagent was purchased from Qingdao Hope Bio-Technology Co., LTD (Qingdao, China). Polyethylene glycol 6000 was bought from Wuxi Yatai Allied Chemicals Co., Ltd. Glucose was from Shanghai Zhanyun Chemical Co., Ltd (Shanghai, China). Vanillin was from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China). Ursolic acid, Folin–Ciocalteu's phenol reagent, gallic acid, rutin hydrate, (+)-catechin, L-ascorbic acid, ABTS, DPPH were purchased from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). (±)-6-Hydroxy 2,5,7,8-tetramethylchromane-2-Carboxylic acid (Trolox) was from Shanghai Aladdin Biochemical Technology Co., Ltd (Shanghai, China). All the reagents were of analytical grade.
7
Fermentation of Tartary Buckwheat with Ganoderma
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Trolox, DPPH, ABTS, p-nitrophenol, p-nitrophenyl-β-D-glucoside (PNPG), DNS, α-amylase, α-glucosidase, xylanase and cellulase were obtained from Macklin Biochemical., Ltd. and Solarbio Biochemical., Ltd. (Shanghai, China). All other chemicals and reagents, such as anhydrous sodium carbonate, potassium ferricyanide, salicylic acid, potassium persulphate, methanol, acetone and anhydrous ethanol used in this study were of analytical grade. Tartary Buckwheat was purchased from Dongfang Shengu Co., Ltd. (Guizhou, China). The fungus Ganoderma lucidum (G10) acquired from Minyuan Fungus (Chongqin, China) was used as a starter for the processing of fermented Tartary buckwheat, stored on PDA agar slant and subcultured every month. Beyond that, Ganoderma lucidum (G10) slants were incubated at 26 °C for 7 days and subsequently preserved in a refrigerator at 4 °C.
8
Recombinant Protein Expression in E. coli
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D-galactose was purchased from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China). ABTS and horseradish peroxidase were purchased from MACKLIN (Shanghai, China). All other chemicals and reagents used were of analytical grade and were purchased from local markets. SDS-PAGE protein standard was from BioRad (Herts, UK). E. coli BL21 Star™ (DE3) strains (Tiangen Biotech, Beijing, China) were used as the prokaryotic expression host.
9
Astaxanthin Antioxidant Extraction Protocol
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Astaxanthin (AST) (pure > 97%) was bought from Aladdin Industrial Corporation (Shanghai, China). ABTS and potassium persulfate were purchased from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). All other solvents including dimethyl sulfoxide (DMSO) and ethanol were supplied by Sinopharm Chemical Reagent Co., Ltd (Shanghai, China).
10
HPLC-purified DNA and miRNA Characterization
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The HPLC-purified
DNA sequences and miRNAs were produced by Shanghai Sangon Biotechnology
Co., Ltd. (Shanghai, China). The details of the sequences can be found
inTable S1 . A solution of deionized water
treated with 0.1% diethylpyrocarbonate (DEPC) was obtained from Beijing
Leagene Biotechnology Co., Ltd. (Beijing, China). The chemicals used
in this study, including potassium chloride (KCl), sodium chloride
(NaCl), magnesium chloride hexahydrate (MgCl2·6H2O), and Triton X-100 (BioUltra), were procured from Sigma-Aldrich.
A 30% concentration of hydrogen peroxide (H2O2) was acquired from Aladdin. The chemicals hemin and ABTS were procured
from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). The
Klenow fragment (3′-5′ exo-) (KF) and 10× NEB buffer
2 (composed of 100 mM Tris-HCl, 500 mM NaCl, 100 mM MgCl2, and 10 mM DTT, with a pH of 7.9) were acquired from New England
Biolabs Co., Ltd. (Beijing, China). The SYBR Green I (10,000×)
and dNTPs Mix (10 mM) were procured from Beijng Solarbio Science &
Technology Co., Ltd. (Beijing, China). The thioflavin T (ThT) compound
was acquired from the Sigma-Aldrich company.
DNA sequences and miRNAs were produced by Shanghai Sangon Biotechnology
Co., Ltd. (Shanghai, China). The details of the sequences can be found
in
treated with 0.1% diethylpyrocarbonate (DEPC) was obtained from Beijing
Leagene Biotechnology Co., Ltd. (Beijing, China). The chemicals used
in this study, including potassium chloride (KCl), sodium chloride
(NaCl), magnesium chloride hexahydrate (MgCl2·6H2O), and Triton X-100 (BioUltra), were procured from Sigma-Aldrich.
A 30% concentration of hydrogen peroxide (H2O2) was acquired from Aladdin. The chemicals hemin and ABTS were procured
from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China). The
Klenow fragment (3′-5′ exo-) (KF) and 10× NEB buffer
2 (composed of 100 mM Tris-HCl, 500 mM NaCl, 100 mM MgCl2, and 10 mM DTT, with a pH of 7.9) were acquired from New England
Biolabs Co., Ltd. (Beijing, China). The SYBR Green I (10,000×)
and dNTPs Mix (10 mM) were procured from Beijng Solarbio Science &
Technology Co., Ltd. (Beijing, China). The thioflavin T (ThT) compound
was acquired from the Sigma-Aldrich company.
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