Bio plex pro human cytokine arrays

Bacterial culture media were purchased from Sigma-Aldrich Inc. (St Louis, MO, USA): Nutrient Broth (Catalogue number 70122) and BSK medium with 6% rabbit serum (Catalogue number B8291). The 96-well culture plates were obtained from Thermo-Fisher Scientific (Waltham, MA, USA): CellStar (Greiner Bio-One, Kremsmünster, Austria, Catalogue number 655-180) for all microbes except Borrelia burgdorferi for which collagen-coated 96-well plates were used (Catalogue number 152038). Other reagents were Crystal Violet (Catalogue number V5265, Sigma-Aldrich (St. Louis, MO, USA)) and CyQUANT VyBrant MTT cell viability assay kit (Catalogue number V13154, Invitrogen, Thermo-Fisher Scientific (Waltham, MA, USA)). Roswell Park Memorial Institute 1640 medium, penicillin–streptomycin 100×, phosphate-buffered saline (PBS), and lipopolysaccharide (LPS) were purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Bio-Plex Pro™ human cytokine arrays were purchased from Bio-Rad Laboratories Inc. (Hercules, CA, USA).

Roswell Park Memorial Institute 1640 medium (Gibco cat. # 11835-030), penicillin–streptomycin 100× (Gibco cat. # 15140-122), fetal bovine serum (Gibco cat. # A38401-01), Dulbecco’s phosphate-buffered saline (PBS, Gibco cat. # 141190-136), lipopolysaccharide (LPS) (Invitrogen cat. # 00-4976-93), monoclonal antibodies CD3-SB702 (clone UCHT1, Invitrogen cat. #67-0038-42), CD56-phycoerythrin (clone CMSSB Invitrogen cat. # 12-0567-42), and CD69-fluorescein isothiocyanate (clone FN50, Invitrogen cat. #11-0699-42) were purchased from Thermo Fisher Scientific (Waltham, MA, USA). CD25-Brilliant Violet 421 (clone 2A3 BD cat. # 564033) and sodium heparin vacutainer tubes (BD cat. # 367878) were purchased from Becton-Dickinson (Franklin Lakes, NJ, USA). Bio-Plex Pro™ human cytokine arrays were purchased from Bio-Rad Laboratories Inc. (Hercules, CA, USA). Interleukin-2 (IL-2) (Sigma cat. # 17908-10KU) was purchased from Sigma-Aldrich Co. (St Louis, MO, USA). Lympholyte Poly (Cedarlane cat. # CL5070) was purchased from CedarLane (Burlington, NC, USA).