Liposomes were prepared following established protocols62 . Briefly, synthetic lipids (Avanti Polar Lipids) were mixed in chloroform, dried under N2, and vacuum dried overnight prior to resuspension in HEPES Buffered Saline with Ca2+ (10 mM HEPES, 0.15 mM NaCl, and 2 mM CaCl2). Resuspended lipids were freeze-thawed five times and extruded nine times through 0.8 µm pore size filters (Whatman). Liposome models tested were: PC liposomes (67.5% PC, 16% Cholesterol, 16.5% Sphingomyelin); PS liposomes (57.5% PC, 10% PS, 16% Cholesterol, 16.5% Sphingomyelin); PA liposomes (57.5% PC, 10% PA, 16% Cholesterol, 16.5% Sphingomyelin); PE liposomes (57.5% PC, 10% PE, 16% Cholesterol, 16.5% Sphingomyelin); PI liposomes (57.5% PC, 10% Soy PI, 16% Cholesterol, 16.5% Sphingomyelin)
Pore size filter
Manufactured by Cytiva
Sourced in Germany
Pore size filters are laboratory equipment designed to separate and purify samples by filtration. They feature a defined pore size that allows the passage of specific components while retaining others, enabling sample clarification and separation.
Automatically generated - may contain errors
4 protocols using pore size filter
1
Preparation of Liposome Models
2
Preparation of Liposome Models
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liposomes were prepared following established protocols62 . Briefly, synthetic lipids (Avanti Polar Lipids) were mixed in chloroform, dried under N2, and vacuum dried overnight prior to resuspension in HEPES Buffered Saline with Ca2+ (10 mM HEPES, 0.15 mM NaCl, and 2 mM CaCl2). Resuspended lipids were freeze-thawed five times and extruded nine times through 0.8 µm pore size filters (Whatman). Liposome models tested were: PC liposomes (67.5% PC, 16% Cholesterol, 16.5% Sphingomyelin); PS liposomes (57.5% PC, 10% PS, 16% Cholesterol, 16.5% Sphingomyelin); PA liposomes (57.5% PC, 10% PA, 16% Cholesterol, 16.5% Sphingomyelin); PE liposomes (57.5% PC, 10% PE, 16% Cholesterol, 16.5% Sphingomyelin); PI liposomes (57.5% PC, 10% Soy PI, 16% Cholesterol, 16.5% Sphingomyelin)
3
Pseudomonas aeruginosa Virulence Factors
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All strains used in this study are listed in Table 1 . CM was prepared as described previously with slight modifications [47 (link)]. Briefly, overnight bacterial cultures in Luria Broth were inoculated 1:50 into RPMI 1640 (Gibco, Life Technologies, Breda, the Netherlands) and incubated at 37°C shaking at 200 rpm. After 5 days, the cultures were centrifuged and supernatants were filter-sterilized through 0.22 μm pore-size filter (Whatman, Dassel, Germany) to obtain CM. Pyocyanin and AprA levels in CM were measured as described previously [63 (link), 64 (link)].
4
Measuring Major Nutrient Concentrations in the Gulf of Mexico
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For major nutrient concentration measurements, 100 ml subsamples of GoM seawater were collected from the same samples that were used in single-cell genomics and respiration analyses. These samples were immediately filtered through a nucleopore track-edge membrane 25 mm, 0.4 µm pore-size filter (Whatman) and stored at −80 °C until analysis of NO3−, NO2−, NH4+ and PO43− on a SEAL AA3 (Seal Analytical Limited). Moreover, triplicate seawater subsamples, 100 ml each, were collected from each field sample and vacuum-filtered onto glass microfiber filters (Whatman, GF/F) within 2 h. Each GF/F filter was extracted in 10 ml of 90% acetone at −20 °C for 24–48 h. The extracts were analysed on the 10-AU fluorometer (Turner Designs) before and after the addition of 50 µl of 10% HCl, and the concentrations of chlorophyll and phaeophytin were determined as described previously79
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!