Paclitaxel

Cell proliferation was assessed using the Cell Counting Kit-8 (CCK-8) assay (Dojindo, Japan) according to the manufacturer’s instructions. Five thousand cells were seeded per well in a 96-well plate and cultured for 72 hours. For the paclitaxel viability assays, 5000 cells per well were plated in 96-well plates for 24 hours and then treated with DMSO or varying doses of paclitaxel (Cell Signaling Technology). After 24 hours of paclitaxel treatment, cell viability was determined using the CCK-8 assay. The relative survival was calculated compared with cells treated with DMSO.

Cells (5 × 10³) were plated in 96-well plates for 24 hours and then treated with DMSO or varying doses of paclitaxel (Cell Signaling Technology, Beverly, MA) as indicated. Cell viability was determined by the Cell Counting Kit-8 (Dojindo, Kumamoto, Japan) 24 hours later. The absorbance was determined at 450 nm using a microplate reader, and the percentage absorbance was calculated against DMSO-treated cells. For cell proliferation assay, cells (2.5 × 10³) were plated in 96-well plates, transfected with 101or NC as described above, and the growth curve of cells, covering a total of four days of culturing, were plotted with the Cell Counting Kit-8 method. For colony formation assay, approximately 500 cells were added to each well of a 6-well culture plate, and each experiment was performed in triplicate. After 12 days of culture at 37°C, cells were fixed with 10% formalin and stained with 10% Giemsa solution. The number of colonies containing ≥ 50 cells was counted under a microscope.