Annexin 5 allophycocyanin apc apoptosis detection kit

The apoptosis of the lung cancer cells was analyzed using an Annexin V-allophycocyanin (APC) apoptosis detection kit (eBioscience; Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol. Briefly, the A549 and NCI-H1975 cells were infected with lentiviruses expressing shCtrl or shDNAJC12 for 24 h. The cells were then harvested (1,000 × g, 5 min, room temperature) and washed with PBS. The cells were subsequently resuspended in a staining buffer to a final density of 1×10⁶ cells/ml. Finally, 100 μl cell suspension was incubated with 5 μl Annexin V-APC at room temperature for 15 min. The stained cells were subjected to flow cytometry using a FACSCalibur flow cytometer (BD Biosciences) and data were analyzed with FlowJo software (version 7.6.1; FlowJo LLC).

The Annexin V-allophycocyanin (APC) apoptosis detection kit (eBioscience, Cat. No. 88–8007, San Diego, CA, USA) was used to detect cell apoptosis. OSCC cells transfected with GBAS-specific shRNA lentivirus or control lentivirus were harvested by trypsinization and resuspended in 1X binding buffer at a final density of 1×106 (link) cells per mL. After centrifugation, the deposits of cells were mixed with 10 μL Annexin V-APC and incubated for 15 min at room temperature. All the samples were assayed and quantified using a Guava EasyCyte Flow Cytometer (Millipore, Hayward, CA, USA).