Anti tiar antibodies

Manufactured by Cell Signaling Technology

Anti-TIAR antibodies are laboratory reagents used to detect and study the TIAR (T-cell Intracellular Antigen-1 Related) protein in biological samples. TIAR is an RNA-binding protein involved in various cellular processes. These antibodies can be utilized in techniques such as western blotting, immunoprecipitation, and immunohistochemistry to identify and analyze the TIAR protein.

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Lab products found in correlation

Protein g sepharose beads, by Cell Signaling Technology (2 mentions) Anti p erk, by Cell Signaling Technology (1 mentions) Thapsigargin tg, by Merck Group (1 mentions) Anti phospho eif2α, by Cell Signaling Technology (1 mentions) Anti cleaved poly adp ribose polymerase cparp, by Cell Signaling Technology (1 mentions) Anti pkd1, by Cell Signaling Technology (1 mentions) Anti atf4 antibody, by Santa Cruz Biotechnology (1 mentions) Anti dnak antibody, by Enzo Life Sciences (1 mentions) Pkc inhibitor g 6983, by Merck Group (1 mentions) Anti bip grp78, by BD (1 mentions) Monoclonal anti α tubulin antibody, by Merck Group (1 mentions) Anti g3bp1, by BD (1 mentions) Anti pkcδ, by BD (1 mentions) Anti eif2α, by Cell Signaling Technology (1 mentions) Pkc inhibitor g 6976, by LC Laboratories (1 mentions)

3 protocols using anti tiar antibodies

Antibody Reagents for Protein Analysis

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Anti-α-tubulin monoclonal antibody was purchased from Sigma-Aldrich. Anti-eIF4γ, anti-BiP/Grp78, anti-PKCδ and anti-G3BP1 monoclonal antibodies were from BD Bioscience; anti-Atg5, anti-Atg16L1, anti-eIF2α, anti-phospho-eIF2α, anti-cleaved caspase7 (cCas7), anti-PERK, anti-cleaved poly(ADP-ribose) polymerase (cPARP), anti-PKD1, anti-phospho-(Ser/Thr) PKD substrates and anti-TIAR antibodies were from Cell Signaling Technology; anti-DAP1, phospho-PKD1 (S738/742) and phospho-PKD1 (S916) antibodies were from Abcam; anti-ATF4 antibody was from Santa Cruz Biotechnology; and anti-GAPDH, anti-PKD2 and anti-PKD3 antibodies were from GeneTex. Anti-DnaK antibody was obtained from ENZO. Anti-SubAB antibody was prepared as previously described (Yahiro et al., 2006 (link)). PKC inhibitor Gö6976 was obtained from LC Laboratories; PKC activator PMA, PKC inhibitor Gö6983, PKD/PKCμ inhibitor CID755673, PKA activator 8Br-cAMP, Thapsigargin (TG), CaM kinase II inhibitor KN-93 were from Sigma Aldrich; and PKA inhibitor 14–22 Amide was from Calbiochem; PKC inhibitor Bisindolylmaleimide II was from ALEXIS Biochemicals; and ROCK II inhibitor Y-27632 was from Cayman Chemical.

Tsutsuki H., Yahiro K., Ogura K., Ichimura K., Iyoda S., Ohnishi M., Nagasawa S., Seto K., Moss J, & Noda M. (2016). Subtilase cytotoxin produced by locus of enterocyte effacement-negative Shiga-toxigenic Escherichia coli induces stress granule formation. Cellular microbiology, 18(7), 1024-1040.

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RIP Assay for Detecting RNA-Protein Interactions

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The RNA immunoprecipitation (RIP) assay was performed using the RiboCluster Profiler RIP assay kit (MBL), anti-TIA-1, anti-TIAR antibodies, and protein G sepharose beads (Cell Signaling) according to the manufacturers’ protocols and our previous study [22 (link)]. Cellular RNA was pulled down with the antibodies and pre-immune rabbit IgG (supplied with the kit). The co-precipitated RNA was purified and sequentially subjected to RT-qPCR to detect TPD52 and β-actin.

Abe Y., Mukudai Y., Kurihara M., Houri A., Chikuda J., Yaso A., Kato K., Shimane T, & Shirota T. (2021). Tumor protein D52 is upregulated in oral squamous carcinoma cells under hypoxia in a hypoxia-inducible-factor-independent manner and is involved in cell death resistance. Cell & Bioscience, 11, 122.

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RNA Immunoprecipitation Assay for TPD52

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The RNA immunoprecipitation (RIP) assay was performed using the RiboCluster Profiler RIP assay kit (MBL), anti-TIA-1, anti-TIAR antibodies, and protein G sepharose beads (Cell Signaling) according to the manufacturers' protocols and our previous study [22] . Cellular RNA was pulled down with the antibodies and pre-immune rabbit IgG (supplied with the kit). The co-precipitated RNA was purified and sequentially subjected to RT-qPCR to detect TPD52 and β-actin.

Abe Y., Mukudai Y., Kurihara M., Houri A., Chikuda J., Yaso A., Kato K., Shimane T., & Shirota T. (2021). Tumor Protein D52 Is Upregulated in Oral Squamous Carcinoma Cells Under Hypoxia in a Hypoxia-inducible-factor-independent Manner and Is Involved in Cell Death Resistance.

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