Piglet lung tissues were isolated and fixed in 10% formaldehyde for 24 h at room temperature. Fixed tissues were dehydrated, embedded in paraffin, and cut in 8-μm sections. For histopathology, sections were rehydrated and stained with hematoxylin and eosin (C0105S, Beyotime) according to the manufacturer’s instructions.
For immunohistochemistry, tissue sections from pig lungs were placed on silane-coated slides, deparaffinized, and rehydrated using decreasing concentrations of ethanol. The sections were then treated with cell and tissue staining kit reagents (CTS005, CTS002, R&D), according to the manufacturer’s instructions, after being incubated with 10 mM citrate buffer for heat-induced antigen retrieval. The following primary antibodies were used at the respective concentrations: rabbit anti-PRRSV N (1:200, GTX129270, GeneTex), rabbit anti-cleaved CASP1 (Asp297) (1:200, 4199s, CST), mouse anti-ASC (1:100, sc-514414, Santa Cruz), and rabbit anti-NLRP3 (1:200, bs-10021R, Bioss).
For immunohistochemistry, tissue sections from pig lungs were placed on silane-coated slides, deparaffinized, and rehydrated using decreasing concentrations of ethanol. The sections were then treated with cell and tissue staining kit reagents (CTS005, CTS002, R&D), according to the manufacturer’s instructions, after being incubated with 10 mM citrate buffer for heat-induced antigen retrieval. The following primary antibodies were used at the respective concentrations: rabbit anti-PRRSV N (1:200, GTX129270, GeneTex), rabbit anti-cleaved CASP1 (Asp297) (1:200, 4199s, CST), mouse anti-ASC (1:100, sc-514414, Santa Cruz), and rabbit anti-NLRP3 (1:200, bs-10021R, Bioss).