Cells were seeded in 6-well ultra-low attachment plates and transfected with siRNAs against the six mesenchymal genes and control siOTP genes. After 48 and 96 h of incubation, the cells were collected and trypsinized into single cell suspensions. The cells were then washed twice with PBS and counted using a hemocytometer. Equal numbers of cells were taken from each siRNA and siOTP genes and then transferred into a 15-ml Falcon tube (BD Biosciences). The cells were resuspended in 100 μl of 1× Annexin binding buffer. Five μl of Pacific Blue Annexin-V (Sigma-Aldrich) and 1 μl of 100 μg/ml Propidium Iodide (Sigma-Aldrich) working solution were added and incubated for 15 min at room temperature. Data were acquired using a BD LSRII flow cytometer (BD Biosciences).
Pacific blue annexin 5
Manufactured by Merck Group
Pacific Blue Annexin-V is a fluorescently-labeled protein used for the detection and quantification of apoptotic cells. It binds to phosphatidylserine, a phospholipid that is exposed on the surface of cells undergoing programmed cell death. The Pacific Blue fluorescent dye is attached to the Annexin-V protein, allowing for the visualization and analysis of apoptotic cells through techniques such as flow cytometry or fluorescence microscopy.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using pacific blue annexin 5
1
Apoptosis Evaluation of Mesenchymal Cells
2
Annexin-V and Propidium Iodide Staining
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were transfected as described above and washed with PBS. Equal numbers of cells were resuspended in 100 μl of Annexin binding buffer, 5 μl of Pacific Blue Annexin-V (Sigma) and 1 μl of 100 μg/ml propidium iodide (Sigma) working solution for 15 min at room temperature. Data were acquired using a BD LSRII flow cytometer (BD Biosciences).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!