All participants underwent SITA fast 24-2 perimetry (Humphrey perimetry, Humphrey Field Analyzer model 750; Carl Zeiss Meditec, Dublin, CA, USA). Minimal criteria for a glaucomatous VF defect were as follows: a Glaucoma Hemifield Test outside normal limits, a pattern standard deviation with P < 5%, or a cluster of ≥3 points in the pattern deviation plot in a single Hemifield (superior or inferior) with P < 0.05, one of which was P < 0.01. Any one of the preceding criteria, if repeatable, was considered sufficient evidence of a glaucomatous VF defect. A reliable VF test was defined as one with fewer than 30% fixation losses, false-positive responses, or false-negative responses.
Humphrey field analyzer model 750
Manufactured by Zeiss
Sourced in United States, Germany
The Humphrey Field Analyzer model 750 is a diagnostic instrument used for conducting visual field tests. It is designed to measure and assess the functionality of the visual field, which is the area of space that can be seen while the eyes are fixed on a central point.
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5 protocols using humphrey field analyzer model 750
1
Glaucoma Diagnosis via SITA Perimetry
2
Optic Disc-Visual Field Relationship
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White-on-white standard automated perimetry was performed using a Humphrey Field Analyzer® Model 750 (Carl Zeiss Meditec, Jena, Germany) and the C-30-2 SITA-standard strategy. We used threshold decibel (dB) values and total deviation as glaucomatous indices for corresponding topographic locations of visual field. We analyzed the relationship between 52 data points of the right eye obtained from the threshold static perimeter and the six corresponding sectors of the optic disc. These points were separated from each other by 6° and from the horizontal and vertical meridians by 3°. A mirror image version was prepared using data obtained from examinations of the left eye. A geometric mean of threshold and a mean of total deviation were calculated for each individual sector.
3
Visual Field Assessment Techniques
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Monocular visual fields were assessed in each eye using the SITA-Standard 24-2 threshold strategy on a Humphrey Field Analyzer Model 750 (Carl Zeiss Meditec, Dublin, CA). Binocular visual fields were also measured using the binocular Esterman test with participants wearing their habitual driving spectacles, if any, as is recommended; the Esterman efficiency score (percentage of points seen) was recorded.
4
Comprehensive Eye Examination Protocol
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All included patients had complete medical records, including age, sex, and comprehensive eye examination results using anterior slit-lamp microscopy, Goldmann applanation tonometry (Haag-Streit, Bern, Switzerland), gonioscopy, dilated fundus examination, AL measurement (IOL Master 700; Carl Zeiss Meditec AG, Jena, Germany), and VF test with a standard 30–2 Swedish interactive threshold algorithm (Humphrey Field Analyzer, Model 750; Carl Zeiss Meditec AG). Color fundus photographs were taken at 45°, centered on the macula, using fundus cameras (Kowa Nonmyd WX; Kowa Inc., Nagoya, Japan; and DRI-OCT, Triton; Topcon, Tokyo, Japan). Optical coherence topography (OCT) images were acquired for all the patients participated (Heidelberg Engineering, Heidelberg, Germany; and DRI-OCT Triton, Topcon). The reliability of the VF test results was monitored as follows: number of fixation losses ≤ 20%, rate of false-negative results ≤ 33%, and rate of false-positive results ≤ 33%.
5
Comprehensive Ophthalmic Assessments in Research
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After medical history information was recorded, comprehensive ophthalmic examinations were performed. Two scan patterns were used for spectraldomain optical coherence tomography (RTVue-100, version 6.1; Optovue, Fremont, CA, USA), including an optic nerve head (ONH) scan centred on the ONH and a ganglion cell complex (GCC) scan centred on the fovea. Visual field testing was performed with SITA fast 24-2 perimetry (Humphrey perimetry, Humphrey Field Analyzer model 750; Carl Zeiss Meditec, Dublin, CA, USA), and the acquired VFI rates were utilized for analysis. Intraocular pressure (IOP) values used for analysis were measured on enrolled day. On the same day, nailfold capillaroscopy and OCTA were examined as follows.
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