Rabbit antibodies against embryonic stem cell-expressed Ras (ERAS) and phospho-parkin (Ser65) were obtained from Biorbyt (St. Louis, MO, USA). Rabbit antibodies against PHB1, optic atrophy 1 (OPA1), LC3, p62, total transcription factor EB (TFEB), phospho-TFEB (Ser211), optineurin (OPTN), PHB1, Tom 20, Calnexin, and HSP90 were obtained from Cell Signaling (Leiden, Netherlands). Rabbit anti-PINK1, goat anti- parkin, and mouse anti-PHB2, c-Myc and β-actin were from Santa Cruz Biotechnology (Dallas, TX, USA). Sheep anti-phospho-PINK1 (Thr257) was purchased from Ubiquigent (Dundee, SC, UK).
Rabbit antibodies
Manufactured by Biorbyt
Sourced in United States
Rabbit antibodies are immunoglobulin proteins produced by rabbits in response to exposure to specific antigens. They are commonly used as research tools in various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Ripa buffer, by Thermo Fisher Scientific (2 mentions)
Calnexin, by Cell Signaling Technology (1 mentions)
Rabbit anti pink1, by Santa Cruz Biotechnology (1 mentions)
Hsp90, by Cell Signaling Technology (1 mentions)
C myc, by Santa Cruz Biotechnology (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
Chemiluminescence system, by Thermo Fisher Scientific (1 mentions)
Polyvinylidene difluoride membrane, by Thermo Fisher Scientific (1 mentions)
Sodium dodecyl sulfate polyacrylamide gel electrophoresis, by Thermo Fisher Scientific (1 mentions)
Horseradish peroxidase conjugated secondary antibody, by Jackson ImmunoResearch (1 mentions)
2 protocols using rabbit antibodies
1
Antibody Sourcing for Mitophagy Pathway
2
Western Blot Analysis of KNL1 and GAPDH
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells and tissues were lysed using RIPA buffer (Thermo Fisher Scientific, Inc.). Lysates were electrophoresed by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (Thermo Fisher Scientific, Inc.) and transferred onto polyvinylidene difluoride membranes (Thermo Fisher Scientific, Inc.). After blocking for 1 hour in 5% skimmed milk, the membranes were incubated overnight at 4°C with rabbit antibodies against KNL1 (dilution, 1:500; Biorbyt Ltd.) and GAPDH (dilution, 1:800; Santa Cruz Biotechnology, Inc., Dallas, TX, USA), washed four times in TBST, and then incubated at room temperature for 1 hour with a horseradish peroxidase-conjugated secondary antibody (dilution, 1:10,000; catalog no., 323-065-021; Jackson ImmunoResearch, Inc., West Grove, PA, USA). Protein bands were visualized using a chemiluminescence system (Thermo Fisher Scientific, Inc.) according to the manufacturer’s protocol. The band intensities were quantified using Image-Quant Software v3.0 (LI-COR Biosciences, Lincoln, NE, USA). This experiment was repeated in triplicate.
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