Hplc grade methanol and water

Manufactured by Thermo Fisher Scientific

Sourced in Canada, United Kingdom

HPLC-grade methanol and water are high-purity solvents specifically designed for use in high-performance liquid chromatography (HPLC) applications. They are formulated to meet the stringent requirements of HPLC instrumentation, providing consistent and reliable performance.

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Lab products found in correlation

10 protocols using hplc grade methanol and water

Methotrexate Pharmacokinetics in Rats

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Long Evans rats (evenly split between females and males) were purchased from Charles River Laboratories (Wilmington, MA) at 1 week of age and were habituated to the vivarium for one week before the experiments. Rats were housed in groups of two or three with a 12/12 hours light/dark cycle and ad lib. access to food (LabDiet 5001) and water. All experiments were approved by the Animal Institute and Use Committee of the Albert Einstein College of Medicine (Bronx, NY) and were conducted following the “Guide for the Care and Use of Laboratory Animals”. The NC3R’s ARRIVE guidelines were followed in the conduct and reporting of all experiments described here.
Methotrexate (USP grade), phosphate buffered saline (PBS) and other chemicals were purchased from Sigma (Saint Louis, MO) unless otherwise stated. Methanol and water (HPLC grade) were obtained from Fisher Scientific (Pittsburgh, PA). All injected solutions were sterilized by filtering through 0.22 μm syringe filters (Millipore, Billerica, MA).

Wen J., Maxwell R.R., Wolf A.J., Spira M., Gulinello M.E, & Cole P.D. (2018). Methotrexate causes persistent deficits in memory and executive function in a juvenile animal model. Neuropharmacology, 139, 76-84.

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Platycodin Compounds Extraction Analysis

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Methanol and water (HPLC grade) were purchased from Fisher Scientific (Fair Lawn, NJ, USA). Deapi-platycoside E, platycodin D3, platycodin D, and polygalacin D were purchased from Target Molecule Corporation (Boston, MA, USA), and platycoside E, platycodin D2, deapi-platycodin D3, and deapi-platycodin D were purchased from Med Chem Express (Monmouth, NJ, USA), whereas 3”-O-acetylplatyconic acid A from ALB Tech (Albany, NY, USA) was used as an external standard for identification. Tubeimoside I was used as the internal standard (100 ppm).

Lee S.J., Kim H.W., Lee S., Kwon R.H., Na H., Kim J.H., Wee C.D., Yoo S.M, & Lee S.H. (2021). Characterization of Saponins from Various Parts of Platycodon grandiflorum Using UPLC-QToF/MS. Molecules, 27(1), 107.

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Mass Spectrometry Imaging of Mouse Brain

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Caffeine, lysine, phenanthrene, phenylalanine, bradykinin, cytochrome c, poly-D-lysine, phenethylamine, and tyramine were purchased from Sigma-Aldrich (St. Louis, MO). HPLC-grade methanol and water were purchased from Fisher Scientific (Nepean, ON, Canada). Mouse brain tissue (C57BL/6J females at 9 weeks) was purchased from The Jackson Laboratory (Bar Harbor, ME) and cryosectioned at a 25-μm thickness with a 550 M Cryostat microtome. The sliced tissues were individually mounted on glass plates and dried for approximately 10 min in a vacuum desiccator prior to imaging. Cell culture medium and reagents including Dulbecco’s Modified Eagle Medium (DMEM), live-cell imaging solution, heat-inactivated fetal bovine serum, horse serum, calf serum, penicillin, streptomycin, and L-glutamine were purchased from Life Technology (Rockville, MD).

Lee J.K., Jansson E.T., Nam H.G, & Zare R.N. (2016). High-Resolution Live-Cell Imaging and Analysis by Laser Desorption/Ionization Droplet Delivery Mass Spectrometry. Analytical chemistry, 88(10), 5453-5461.

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Quantitative Peptidomics via EF-LESA

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Leucine enkephalin,
angiotensin II,
and N-acetylarginine were purchased from Fisher Scientific
U.K. Ltd. (Loughborough, U.K.). Individual stock solutions (LE, 180 pmol/μL; AT, 956 pmol/μL;
and NAA, 920 pmol/μL were prepared in a solvent
mixture A, consisting of pure water). Compounds were chosen because
they were soluble in H₂O and provide a good ESI response.
Solutions used in experiments and for construction of the calibration
graphs were prepared at the desired concentration by serial dilution
in water from the stock solution with further dilution, after surface
sampling, with 50:50 water (with 0.1% formic acid):methanol solution
(solvent mixture B). A solvent mixture of water with 0.1% formic acid
was used as the EF-LESA probe mixture (solvent mixture C). HPLC-grade
methanol and water were purchased from Fisher Scientific U.K. Ltd.,
and formic acid was supplied by Sigma Aldrich Ltd. (Gillingham, Dorset,
U.K.).

Brenton A.G, & Godfrey A.R. (2014). Electro-Focusing Liquid Extractive Surface Analysis (EF-LESA) Coupled to Mass Spectrometry. Analytical Chemistry, 86(7), 3323-3329.

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Cyclodextrin-based Econazole Ocular Delivery

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2′-hydroxypropyl-α-cyclodextrin and 2′-hydroxypropyl-β-cyclodextrin and Econazole nitrate were purchased from Sigma-Aldrich Company Ltd. (Poole, UK). HPLC-grade methanol and water, trifluoroacetic acid (TFA) and phosphate-buffered saline (PBS) tablets were purchased from Fisher Scientific UK Ltd. (Loughborough, UK). Acuvue TruEye^® daily disposable contact lenses (Johnson and Johnson, Ireland, UK) were provided by Cardiff University Eye Clinic (Cardiff, UK). Porcine eyeballs were supplied by a local abattoir having been enucleated immediately post-mortem.

Wong A., Fallon M., Celiksoy V., Ferla S., Varricchio C., Whitaker D., Quantock A.J, & Heard C.M. (2022). A Composite System Based upon Hydroxypropyl Cyclodextrins and Soft Hydrogel Contact Lenses for the Delivery of Therapeutic Doses of Econazole to the Cornea, In Vitro. Pharmaceutics, 14(8), 1631.

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Nanoparticle Fabrication and Characterization

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1,2-Dioleoyl-sn-glycero-3-phosphoethanolamine
(DOPE: 18:1 (Δ9-Cis) was purchased from Avanti Polar Lipids
(Alabaster, AL). N-(3-(Dimethylamino)propyl)-N′-ethylcarbodiimide (EDC), N-hydroxysuccinimide
(NHS), 4-pentynoic acid, copper(II) sulfate pentahydrate, l-ascorbic acid, bathophenanthroline, Triton X-100, and sodium bicarbonate
were obtained from Sigma–Aldrich Chemical Co. (St. Louis, MO).
BCA Protein Assay Reagent and HPLC grade methanol and water were purchased
from Fisher Scientific (Pittsburgh, PA). Mouse mammary carcinoma (4T1)
cells were acquired from ATCC (Manassas, VA). Roswell Park Memorial
Institute (RPMI) medium 1640, 1× phosphate buffered saline (PBS),
fetal bovine serum (FBS), trypsin, and penicillin–streptomycin
were all purchased from Mediatech, Inc. (Manassas, VA). Plasmocin
was obtained from InvivoGen (San Diego, CA). Hoechst 33342 nucleic
acid stain, and DID (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine
perchlorate) was obtained from Life Technologies (Carlsbad, CA). Azide-Fluor
545 was purchased from KeraFAST, Inc. (Boston, MA). Formalin was purchased
from JT Baker (Center Valley, PA). Sepharose CL-4B was purchased from
GE Healthcare (Uppsala, Sweeden). Sequencing grade trypsin was obtained
from Promega Corporation (Madison, WI).

Smyth T., Petrova K., Payton N.M., Persaud I., Redzic J.S., Graner M.W., Smith-Jones P, & Anchordoquy T.J. (2014). Surface Functionalization of Exosomes Using Click Chemistry. Bioconjugate Chemistry, 25(10), 1777-1784.

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Comprehensive Microcystin Quantification

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LC-MS grade acetonitrile, water and formic acid and HPLC-grade methanol and water were purchased from Fisher (ThermoFisher, UK). Reference toxin standards of microcystins (MC-LR, MC-RR, MC-YR, MC-WR, MC-LW, MC-LA, MC-LY, MC-LF, MC-HtyR, MC-HiLR, [Asp3]-MC-LR/[Dha7]-MC-LR) and NOD ≥95% were as per Enzo Life Sciences (Exeter, UK) (Fig. S1). A certified standard of [Dha7]-MC-LR and a pre-certified freeze-dried matrix reference material of blue-green algae (RM-BGA, Lot 201301) containing a range of MCs were purchased from the Institute of Biotoxin Metrology, National Research Council Canada (Ontario, Canada).
A mixed stock solution was prepared by combining aliquots of each toxin to give a final concentration of 327 μg/L. For external calibration, a seven point calibration curve was prepared by serial dilution with methanol/water (1:1, v/v) in the range of 0.33–327 μg/L for each toxin and stored at – 18 °C. A quality control reference material (RM-BGA, National Research Council, Halifax, Canada) was prepared, with toxins extracted in the supernatant after 28 mL of methanol/water (1:1, v/v) + 0.1% acetic acid were added to RM-BGA (280 mg) and subsequent centrifugation (4500

\times

g; 10 min).
Shellfish diet 1800 (approximately 7.4 × 10¹¹ cells/mL) was purchased from ReedMariculture Inc., (US) and dilutions were made in water/seawater (10:0.86, v/v).

Camacho-Muñoz D., Waack J., Turner A.D., Lewis A.M., Lawton L.A, & Edwards C. (2021). Rapid uptake and slow depuration: Health risks following cyanotoxin accumulation in mussels?. Environmental Pollution (Barking, Essex : 1987), 271, 116400.

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Pharmaceutical Compound Characterization

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Aceclofenac, carprofen, diclofenac, ibuprofen, indomethacin, naproxen, and salicylic acid were from Sigma-Aldrich (Oakville, ON, Canada). HPLC-grade methanol and water were from Fisher Scientific (Ottawa, ON, Canada). All other reagents were from VWR International Ltd (Mississauga, ON, Canada) unless otherwise specified.

Price H.R., Lai D., Kim H., Wright T.E., Coughtrie M.W, & Collier A.C. (2020). Detection and quantitation of non-steroidal anti-inflammatory drug use close to the time of birth using umbilical cord tissue. Toxicology Reports, 7, 1311-1318.

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Quantification of Emodin in Rhubarb Extract

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An Agilent 1100 high performance liquid chromatography system, including a programmable solvent delivery pump, autosampler, and diode-array UV detector, was used for determination of emodin. Detection was set at 437 nm for emodin (6-methyl-1,3,8trihydroxyanthraquinone) analysis. Emodin separation was carried out using a Kinetex 5 um XBC18 100A 250 × 4.6mm column (Phenomenex, Torrance, CA). HPLC-grade methanol and water were used as reagents (Fisher Scientific). Isocratic separation was performed with a methanol/water (70:30, v/v) mobile phase at a flow rate of 0.8 mL/min. A stock emodin standard of 100 μg/mL was used to develop a seven-point calibration curve following serial dilution with methanol. Rhubarb extract was prepared in methanol at 100 mg/L. Each sample or standard was then sonicated for 30 minutes prior to triplicate injection via HPLC. A 30 μL aliquot of all samples or standards were injected directly into the HPLC system for quantitation. Emodin was identified in unknown samples by retention time matching between standards and unknowns, and the data was expressed in μg/mL.

Evans L.W., Bender A., Burnett L., Godoy L., Shen Y., Staten D., Zhou T., Angermann J.E, & Ferguson B.S. (2020). Emodin and emodin-rich rhubarb inhibits histone deacetylase (HDAC) activity and cardiac myocyte hypertrophy. The Journal of nutritional biochemistry, 79, 108339.

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Preparation of Apolipoprotein Mb Variants

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Plasmids encoding most of the apoMb variants used in this study were prepared at the Institute of Protein Research in Pushchino^{54 (link)} while others were a generous gift from Drs. Jane Dyson and Peter Wright (The Scripps Research Institute). Ultra-pure urea was from MP Biochemicals, Inc. (Solon, OH). Trifluoroacetic acid was from Sigma-Aldrich (St. Louis, MO). Sodium acetate, acetonitrile, HPLC grade methanol and water, and other chemicals were from Fisher Scientific (Pittsburg, PA). Purchased reagents were used as received.

Mizukami T., Xu M., Fazlieva R., Bychkova V.E, & Roder H. (2018). Complex Folding Landscape of Apomyoglobin at Acidic pH Revealed by Ultrafast Kinetic Analysis of Core Mutants. The journal of physical chemistry. B, 122(49), 11228-11239.

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