Ammonium persulfate psa

The following chemical reagents were used in this work: tris-hydroxymethylaminomethane (Tris, 111TRIS01KG) glycine (Q5778), dithiothreitol (020909), and Coomassie Brilliant Blue R-250 (821616) were from MP Biomedicals (Eschwege, Germany); KCl (1101–1.0), K₂HPO₄ (2981C270), KH₂PO₄ (1138B228), N,N,N’,N’-tetramethylethylenediamine (TEMED, L-0847–10,0), and sodium dodecyl sulfate (SDS, 0116B026) were purchased from Helicon (Moscow, Russia); acrylamide (DE790612.0500) and N,N’-methylenebisacrylamide (DE110269.0050) were from Dia-M (Moscow, Russia); Triton X-100 (Am-0694) and ammonium persulfate (PSA) were from Sigma (A3678St, Louis, MO, USA); protein molecular weight markers, PageRuler, 10–200 kDa (Thermo Fisher Scientific Baltics, Vilnius, Lithuania, 26630); hydrogen peroxide (H₂O₂) was from Sigma-Aldrich (St. Louis, MO, USA). An ELISA kit was used for the determination of the IgG subclass (A-8672, VECTOR-BEST, PO BOX 492, Novosibirsk, Russia). Two ELISA kits were used for the evaluation of the antibodies against the S-protein (5501 SARS-CoV-2-IgG-EIA-BEST VECTOR-BEST, PO BOX 492, Novosibirsk, Russia) and N-protein (5507 SARS-CoV-2-Ag-EIA-BEST VECTOR-BEST, PO BOX 492, Novosibirsk, Russia).

Hybrid polyacrylamide hydrogels (PAAm) were synthesized with the modified and unmodified inorganic phases. In all cases, the following procedure was applied: for the syntheses, the inorganic phases were used on a dry basis. The sample was pulverized with a mortar. The necessary amount of the inorganic phase (0.1%, 1%, or 10% of the total mass of the monomer) was dispersed in a volume of deionized water in test tubes, which was placed under ultrasonic stirring for 15 min. Subsequently, the respective amounts of the monomers and crosslinking agent (N, N′-methylenebisacrylamide (NMBA), manufactured by Sigma-Aldrich (Saint Louis, MO, USA)) were dissolved and placed for 15 min in ultrasound to homogenize the system. Finally, the initiator, ammonium persulfate (PSA) manufactured by Sigma-Aldrich (Saint Louis, MO, USA), was added. Once the mixture was homogenized, it was placed under ultrasonic stirring at 55 °C for 3 h. At the end of the synthesis, the hydrogels were purified with successive washes of deionized water (the water was changed until pH = 7 was obtained) and then dried at ambient conditions. Table 2 shows the nomenclature assigned to each product and the conditions used in the synthesis.