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Seahorse xf real time atp

Manufactured by Agilent Technologies

The Seahorse XF real-time ATP is a laboratory instrument designed to measure cellular energy metabolism in real-time. It provides quantitative data on cellular adenosine triphosphate (ATP) production.

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2 protocols using seahorse xf real time atp

1

Agilent Seahorse XF ATP Assay

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Experiments were performed in Agilent XF DMEM medium (103,576–100) as per protocol provided by Agilent Seahorse XF real-time ATP rate assay kit user guide. LDLc or HDLc or vehicle was injected through port A and allowed to incubate for 45–50 min, followed by injection of oligomycin, and rotenone & antimycin A in ports B, C, and D respectively in each well. OCR and ECAR were measured by using a Seahorse XFe24 analyzer, Agilent (CA, USA). Data was analyzed in seahorse wave desktop software (CA, USA).
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2

Agilent Seahorse XF ATP Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Experiments were performed in Agilent XF DMEM medium (103,576–100) as per protocol provided by Agilent Seahorse XF real-time ATP rate assay kit user guide. LDLc or HDLc or vehicle was injected through port A and allowed to incubate for 45–50 min, followed by injection of oligomycin, and rotenone & antimycin A in ports B, C, and D respectively in each well. OCR and ECAR were measured by using a Seahorse XFe24 analyzer, Agilent (CA, USA). Data was analyzed in seahorse wave desktop software (CA, USA).
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