Cells were incubated with Fc blocking antibody in 0.5% BSA at 4°C for 30 min. Cells were then immunostained with the following antibodies: FITC-conjugated anti-CD11b, PE/Cy7-conjugated anti-CD11c, APC-conjugated anti-CD45 and PE-conjugated anti-CD207 primary antibodies, and isotype-matched control antibodies (eBioscience, San Diego, CA). Stained cells were analysed using the LSRII flow cytometer (BD Biosciences, San Jose, CA) and Summit v4.3 software (Dako Colorado, Fort Collins, CO).
Pe cy7 conjugated anti cd11c
Manufactured by Thermo Fisher Scientific
PE/Cy7-conjugated anti-CD11c is a fluorescently labeled antibody that binds to the CD11c surface antigen expressed on dendritic cells and other myeloid cells. The PE/Cy7 fluorescent label allows for the detection and analysis of CD11c-positive cells using flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Isotype matched control antibody, by Thermo Fisher Scientific (1 mentions)
Fitc conjugated anti cd11b, by Thermo Fisher Scientific (1 mentions)
Lsrii flow cytometer, by BD (1 mentions)
Creative suite, by Adobe (1 mentions)
Fitc conjugated anti f4 80, by Thermo Fisher Scientific (1 mentions)
Pe conjugated cd11b, by BD (1 mentions)
Lsrfortessa flow cytometer, by Tree Star (1 mentions)
2 protocols using pe cy7 conjugated anti cd11c
1
Immunophenotyping of Myeloid Cells
2
Multiparameter Flow Cytometry of Macrophages
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
BMDMs were resuspended and stained with PE-Cy7-conjugated anti-CD11c (eBioscience clone N418, 25-0114), FITC-conjugated anti-F4/80 (eBioscience clone BM8, 11-4801), and PE-conjugated CD11b (BD Pharmingen, San Jose, CA, clone M1/70 553311). BD and eBioscience antibodies to CD45 were used as single-stained controls. Data were collected on a BD LSRFortessa flow cytometer running FACSDiva software and analyzed in FlowJo (TreeStar, Ashland, OR). Figures were made in Adobe Creative Suite (San Jose, CA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!