Flow cytometry was performed as per manufacturer’s protocol asked. BD Multitest CD3/CD8/CD45/CD4 reagent and BD Multitest CD3/CD16+CD56/CD45/CD19 reagent (BD Bioscience, CA, USA) were used to measure the lymphocyte percentage. Human Th1/Th2/Th17 Phenotyping Kit (Cell-Genebio, China) was used for the determination of IL-2, IL-4, IL-6, IL-10, TNF-α, IFN-γ, and IL-17a. All tests were performed on FACSCalibur (BD Biosciences, USA) instruments. The software FlowJo (LLC, version 10.6.0) was used for the data analysis.
Multitest cd3 cd16 cd56 cd45 cd19 reagent
Manufactured by BD
Sourced in United States
The BD Multitest CD3/CD16+CD56/CD45/CD19 reagent is a flow cytometry reagent used for the identification and enumeration of T cells, natural killer cells, B cells, and overall leukocytes in human whole blood samples.
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Lab products found in correlation
2 protocols using multitest cd3 cd16 cd56 cd45 cd19 reagent
1
Lymphocyte and Cytokine Profiling by Flow Cytometry
2
Whole Blood Immunophenotyping by Flow Cytometry
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Total 227 cases collected between July 2016 and September 2019 and performed by flow cytometry. The manipulation was referred to BD operating instruction.
For each sample, two trucount tubes labeled with the simple identification number such as letters A and B to distinguish from each other.
20 μL of BD Multitest CD3/CD4/CD8/CD45 reagent was pipetted into the bottom of each tube marked A.
20 μL of BD Multitest CD3/CD16 + CD56/CD45/CD19 reagent were pipetted into the bottom of each tube marked B.
50 μL of well-mixed and anticoagulated whole blood was pipetted into the bottom of every tube using the method of reverse pipetting.
Then vortexed gently to mix, incubate for 15 min in dark at room temperature.
Finally, 450 μL of 1× BD Multitest IMK kit lysing solution was pipetted into every tube (Dilute the 10× concentrate 1:10 with room temperature deionized water). The solution was vortexed gently to mix and incubated for 15 min in dark at room temperature.
Samples were analyzed on the flow cytometer.
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