Pkcζ antibody

Manufactured by Cell Signaling Technology

Sourced in United States

The PKCζ Antibody is a specific antibody that recognizes the protein kinase C zeta (PKCζ) enzyme. PKCζ is a member of the protein kinase C family and plays a role in various cellular processes, such as cell growth, differentiation, and survival. This antibody can be used for the detection and analysis of PKCζ in various experimental applications.

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3 protocols using pkcζ antibody

Protein Kinase Signaling Pathway Analysis

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The following antibodies were purchased from Cell Signaling (Danvers, MA, USA): JAK1 Antibody (#3332, 130kDa), Phospho-JAK2 (Tyr1007/1008) Antibody (#3771, 125kDa), β-Actin Antibody (#4967, 45kDa), Phospho-STAT1 (Tyr701) (58D6) Rabbit mAb (#9167, 84,91kDa), Phospho-STAT2 (Tyr690) Antibody (#4441, 113kDa), Phospho-STAT3 (Tyr705) Antibody (#9131, 79,86kDa), Phospho-STAT5 (Tyr694) Antibody (#9351, 90kDa), Phospho-STAT6 (Tyr641) Antibody (#9361, 110kDa), Ras Antibody (#3965, 21kDa), p44/42-MAPK (Erk1/2) Antibody (#9102, 42,44kDa), GSK-3β (27C10) Rabbit mAb (#9315, 46kDa), MMP-9 Antibody (#3852, 84,92kDa), Phospho-PKCα/β II (Thr638/641) Antibody (#9375, 80,82kDa); PKCα Antibody (#2056, 80kDa); Phospho-PKC (pan) (βII Ser660) Antibody (#9371, 78 a 85kDa); Phospho-PKD/PKCμ (Ser744/748) Antibody (#2054, 115kDa); PKD/PKCμ Antibody (#2052, 115kDa); PKCδ Antibody (#2058, 78kDa); Phospho-PKCδ/θ (Ser643/676) Antibody (#9376, 78kDa), PKCζ Antibody (#9372, 78kDa); PLCγ1 Antibody (#2822, 155kDa); anti-mouse, anti-rabbit and anti-goat IgGs antibodies. From Abcam (Cambridge, MA, USA): anti-PKC antibody (ab59363) was purchased. PepChip1-Kinomics^™ slides were obtained from Pepscan Presto BV (Lelystad, the Netherlands).
WP1066 (#573097; C₁₇H₁₄BrN₃O) and Tofacitinib (#PZ0017; C₁₆H₂₀N₆O · C₆H₈O₇) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Nascimento A.S., Peres L.L., Fari A.V., Milani R., Silva R.A., da Costa Fernandes C.J., Peppelenbosch M.P., Ferreira-Halder C.V, & Zambuzzi W.F. (2017). Phosphoproteome profiling reveals critical role of JAK-STAT signaling in maintaining chemoresistance in breast cancer. Oncotarget, 8(70), 114756-114768.

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Western Blot Analysis of PKCζ Expression

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Cells were lysed in a cold buffer containing phosphatase inhibitors and the protein concentration was determined with a bicinchoninic acid assay (Pierce, Thermo Scientific, Rockford, IL, USA) according to the manufacturer's protocol. In total, 20 μg of denatured protein in Laemmli's loading buffer was run on a 10% acrylamide gel and subsequently blotted onto a nitrocellulose membrane (Schleicher & Schuell, Dassel, Germany) in cold methanol-containing blotting buffer. C-terminally binding PKC ζ (H-1) antibody was ordered from Santa Cruz Biotechnology (Santa Cruz, CA, USA; sc-17781), N-terminally binding (personal communication with the manufacturer) PKCζ Antibody from Cell Signaling Technology (Danvers, MA, USA; #9372), β-actin from Abcam (Cambridge, UK) (ab6276), and a fluorescently labeled secondary IRDye antibody from LI-COR Biosciences (Lincoln, NE, USA). Blots were scanned using an Odyssey Infrared Imaging System (LI-COR Biosciences).

Hartsink-Segers S.A., Beaudoin J.J., Luijendijk M.W., Exalto C., Pieters R, & Den Boer M.L. (2014). PKCζ and PKMζ are overexpressed in TCF3-rearranged paediatric acute lymphoblastic leukaemia and are associated with increased thiopurine sensitivity. Leukemia, 29(2), 304-311.

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Baicalin Regulates Apoptosis and Metastasis

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Agents and antibodies included: baicalin (Sigma-Aldrich, Cat# 572667), TUNEL kit (Roche, Cat# 11684795910), PKCζ antibody (Cell Signaling Tech, Cat#9372, 1: 4000), STAT3 antibody (Cell Signaling Tech, Cat#4368, 1: 2000), phosphorylated STAT3 antibody (p-STAT3, Cell Signaling Tech, Cat#8119, 1: 2000), Survivin antibody (Abcam, Cat#ab76424, 1: 4000), matrix metalloproteinase (MMP)2 antibody (Abcam, Cat#ab37150, 1: 4000), MMP9 antibody (Abcam, Cat#ab38898, 1: 4000), Histone H3 antibody (Abcam, Cat#ab8580, 1: 4000), and GAPDH antibody (Sigma-Aldrich, Cat#G9545, 1: 6000).

Wang Q., Xu H, & Zhao X. (2018). Baicalin Inhibits Human Cervical Cancer Cells by Suppressing Protein Kinase C/Signal Transducer and Activator of Transcription (PKC/STAT3) Signaling Pathway. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 24, 1955-1961.

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