GSH-Glo Glutathione Assay kit (Promega) was utilized to measure cellular GSH levels. After treatment with various agents, the medium was removed from the wells. Then cells were incubated with 100 μl mixed GSH-Glo reagent for 30 min and then 100 μl Luciferin Detection Reagent for another 15 min. Luminescent signals were detected using a Fluoroskan luminescence scanner (Thermo Fisher, Rockford, IL, USA).
Fluoroskan luminescence scanner
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Fluoroskan luminescence scanner is a laboratory instrument designed to measure luminescence signals from samples. It is capable of detecting and quantifying various luminescent molecules, including those used in biological assays. The core function of the Fluoroskan is to provide accurate and reliable luminescence measurements to support research and analysis activities in a laboratory setting.
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Lab products found in correlation
4 protocols using fluoroskan luminescence scanner
1
Cellular Glutathione Quantification
2
GSH Quantification in HCT116 Cells
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GSH was measured using the GSH-Glo™ glutathione assay kit (cat. no. V6911; Promega Corporation) according to the manufacturer's instructions. Briefly, HCT116 cells were seeded at a density of 2×104 cells/well into 96-well opaque plates and treated with the indicated siRNA (si155 or si156). After removing the medium, the cells were incubated in 100 µl mixed GSH-Glo™ reagent for 35 min at room temperature and subsequently in 100 µl reconstituted Luciferin Detection Reagent for 15 min at room temperature. Luminescent signals were detected using a Fluoroskan luminescence scanner (Thermo Fisher Scientific, Inc.).
3
Measuring Cellular Glutathione and ROS
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Cellular glutathione levels were measured by a GSH-Glo™ glutathione assay kit (Promega, Madison, WI, USA). After different treatments, cells were incubated in 100 μl mixed GSH-Glo™ Reagent for 30 min, and then 100 μl reconstituted Luciferin Detection Reagent was added for 15 min. Luminescence signals were detected using a Fluoroskan luminescence scanner (Thermo Fisher, Rockford, IL, USA).
Cellular ROS levels were measured by the Reactive Oxygen Species Assay Kit (Beyotime, China). Cells were incubated with 10 μM 2′,7′-dichlorodihydro uorescein diacetate (DCFH-DA) and the supernatants of differently treated cell lysates for 30 min at room temperature. The uorescence intensity of the cells was analyzed by FCM (BD FACSCalibur; BD Biosciences).
Cellular ROS levels were measured by the Reactive Oxygen Species Assay Kit (Beyotime, China). Cells were incubated with 10 μM 2′,7′-dichlorodihydro uorescein diacetate (DCFH-DA) and the supernatants of differently treated cell lysates for 30 min at room temperature. The uorescence intensity of the cells was analyzed by FCM (BD FACSCalibur; BD Biosciences).
4
Measuring Cellular Glutathione and ROS
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cellular glutathione levels were measured by a GSH-Glo™ glutathione assay kit (Promega, Madison, WI, USA). After different treatments, cells were incubated in 100 μl mixed GSH-Glo™ Reagent for 30 min, and then 100 μl reconstituted Luciferin Detection Reagent was added for 15 min. Luminescence signals were detected using a Fluoroskan luminescence scanner (Thermo Fisher, Rockford, IL, USA).
Cellular ROS levels were measured by the Reactive Oxygen Species Assay Kit (Beyotime, China). Cells were incubated with 10 μM 2′,7′-dichlorodihydro uorescein diacetate (DCFH-DA) and the supernatants of differently treated cell lysates for 30 min at room temperature. The uorescence intensity of the cells was analyzed by FCM (BD FACSCalibur; BD Biosciences).
Cellular ROS levels were measured by the Reactive Oxygen Species Assay Kit (Beyotime, China). Cells were incubated with 10 μM 2′,7′-dichlorodihydro uorescein diacetate (DCFH-DA) and the supernatants of differently treated cell lysates for 30 min at room temperature. The uorescence intensity of the cells was analyzed by FCM (BD FACSCalibur; BD Biosciences).
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