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Mirna mimic hsa mir 15a 5p and hsa mir 16 5p

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The miRNA mimic (hsa-miR-15a-5p and hsa-miR-16-5p) is a synthetic double-stranded RNA molecule designed to mimic the function of the endogenous human microRNA (miRNA) sequences hsa-miR-15a-5p and hsa-miR-16-5p. These mimics can be used for research purposes to study the biological roles and regulatory effects of these specific miRNA species.

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Lab products found in correlation

Oligofectamine, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

MiR-15a-5p mimic Hsa-miR-16 MiR-16-5p MiRNA mimics MiR-16

2 protocols using mirna mimic hsa mir 15a 5p and hsa mir 16 5p

1

Overexpression of miR-15a and miR-16 in REC

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REC were transfected with miRNA mimic (hsa-miR-15a-5p and hsa-miR-16-5p) (Invitrogen, Carlsbad, CA) using Oligofectamine (Invitrogen) following manufacturer instructions. miR-transfection was performed 48 h before cell harvest. A final concentration of 30 nM was used when transfected separately (miR-15a and miR-16), and 15 nM was used in combination (miR-15a + miR-16). Additionally, a 30-nM Mimic Negative Control (Invitrogen) was transfected into REC cultured in high glucose as a control. Other control groups, normal glucose (NG) and high glucose (HG), were treated with Oligofectamine only. The levels of miRNA overexpression were verified using quantitative reverse transcription polymerase chain reaction and real-time PCR.
Ye E.A., Liu L., Jiang Y., Jan J., Gaddipati S., Suvas S, & Steinle J.J. (2016). miR-15a/16 reduces retinal leukostasis through decreased pro-inflammatory signaling. Journal of Neuroinflammation, 13, 305.
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2

Transfection of miRNA Mimics in REC

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As described in our previous study (Ye & Steinle, 2015 (link)), transfection was performed on REC with miRNA mimic (hsa-miR-15a-5p and hsa-miR-16-5p) (Invitrogen, Carlsbad, CA) using Oligofectamine (Invitrogen) following manufacturer’s instructions. Cells were transfected 48 h before protein extraction. A final concentration of 30 nM was used when transfected separately (miR-15a and -16) and 15 nM was used in combination (miR-15a + miR-16). A 30 nM Mimic Negative Control (Invitrogen) was transfected into REC cultured in high glucose as a control. Other control groups, normal glucose (NG) and high glucose (HG), were treated with Oligofectamine only.
Ye E.A., Liu L, & Steinle J.J. (2017). miR-15a/16 inhibits TGF-beta3/VEGF signaling and increases retinal endothelial cell barrier proteins. Vision research, 139, 23-29.
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