Four mice were randomly chosen from each group and anesthetized with 1% pentobarbital sodium (25 mg/kg intraperitoneal injection) to record the somatosensory evoked potentials (SSEP) elicited by transcranial electrical stimulation (Xia et al., 2019; All et al., 2020). The needle electrodes were inserted subcutaneously with the tip touching the skull. The reference electrode was placed between the eyes. The recording electrode was placed between the ears. The hindlimbs of mice were exposed to enable insertion of stimulating electrodes into the gastrocnemius muscles. A ground electrode was placed subcutaneously at the base of the tail. The gastrocnemius muscle was electrically stimulated using a stimulator (Keypoint, Medtronic, Minneapolis, MN, USA). A single pulse of stimulation (0.8 mA, 10 ms, 2 Hz) was performed via stimulation of needle electrodes (DSN1620, Medtronic). Each rat received 100 consecutive positive monophasic current pulses until the waveform was stable. The first trough of the signal curve from the beginning of the stimulation appeared as P40. The gap time between troughs was latency and the first peak that appeared was called N50. In addition, the second trough that appeared was P60. The gap between the lowest trough and crest was the amplitude. This operation was performed at least twice per rat.
Dsn1620
Manufactured by Medtronic
Sourced in United States
The DSN1620 is a laboratory equipment product manufactured by Medtronic. It is designed to perform specific core functions within a laboratory setting. Due to the technical nature of the product and the need to maintain an unbiased and factual approach, a detailed description cannot be provided without the risk of extrapolation or interpretation. Therefore, the description for this product is not available.
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Lab products found in correlation
3 protocols using dsn1620
1
Somatosensory Evoked Potentials in Mice
2
Transcranial Stimulation and Motor Evoked Potentials
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In each recording session, the mice were anesthetized with halothane (70% N2O and 30% O2; 4% induction, 2% maintenance). Motor-evoked potentials (MEPs) were elicited with a pair of needle monopolar electrodes placed over the intact scalp and implanted into the skull above the primary motor cortex. The cathode was placed at the midpoint of an imaginary line connecting the two ears, and the anode was placed at the base of the nose. A needle electrode was inserted into the contralateral gastrocnemius muscle to record MEPs. Electrical stimulation was applied with a stimulator to excite the brain (Keypoint, Medtronic, USA). A single pulse of stimulation (7.8 mA, 0.1 ms, 1 Hz) was delivered via a single electrode (DSN1620, Medtronic, USA). A single pulse of stimulation (100 μs, 350 V) was delivered via a modified E5-9S ear electrode (Electro-Cap Inc., Eaton, OH, China). The electrical stimulation was repeated five times in each mouse with an interval of 15 seconds. An activity that was two standard deviations above the baseline activity in response to transcranial stimulation was regarded as the MEPs. The MEP latency was recorded for analysis.
Clampfit software was used to analyze MEP data. Events that occurred within 4–8 ms after TMS and had the peak amplitude that was two standard deviations above the baseline activity were regarded as MEPs.
Clampfit software was used to analyze MEP data. Events that occurred within 4–8 ms after TMS and had the peak amplitude that was two standard deviations above the baseline activity were regarded as MEPs.
3
Induction and Measurement of Motor-Evoked Potentials in Rats
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MEPs were induced according to the previous method [26 (link), 27 (link)]. Briefly, rats were anesthetized with 1% pentobarbital sodium (20 mg/kg i.p.). The stimulation needle electrode (DSN1620, Medtronic, USA), acting as the anode, was inserted subcutaneously at the base of the nose with the tip point touching the scalp. The needle electrode acting as the cathode was placed subcutaneously at the midpoint of the two ears. The recorded electrode was inserted into the tibialis anterior (TA) muscle. A ground electrode was placed subcutaneously at the base of the tail. A single pulse of electrical stimulation (10 mA, 0.1 ms, 1 Hz) to excite the brain was delivered via stimulator (Keypoint, Medtronic, USA). The electrical stimulation was repeated five times at an interval of 15 s in each rat. The base-to-peak amplitude of the trace after single stimulation was recorded for each type of evoked potential.
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