Pngase f with 10 g7 reaction buffer 0.5 m sodium phosphate

HPLC grade methanol, isopropanol, and acetic acid were procured from Fisher Scientific (Pittsburgh, PA). Acetonitrile (ACN) and acetone were acquired from JT Baker (Phillipsburg, NJ). HPLC grade water and sodium hydroxide were purchased from Mallinckrodt Chemicals (Phillipsburg, NJ). Borane-ammonia complex, sodium hydroxide beads, dimethyl sulfoxide (DMSO), iodomethane, trifluoroacetic acid (TFA), and formic acid (FA) were purchased from Sigma-Aldrich (St. Louis, MO). PNGase F with 10×G7 reaction buffer (0.5 M sodium phosphate) was obtained from New England Biolabs (Ipswich, MA). AminoxyTMT^™ reagent was obtained from Thermo Fisher Scientific (Rockford, IL).
RNase B and fetuin from bovine serum were purchased from Sigma-Aldrich (St. Louis, MO). Human blood serum from disease free and patients with Barrett’s disease (BE), high-grade dysplasia (HGD) and esophageal adenocarcinoma (EAC) was provided by Dr. Zane Hammoud (Henry Ford Hospital, Detroit, MA). All cell lines were purchased from American Type Culture Collection (ATCC; Manassas, VA) except for the subline MDA-MB-231Br, which was provided by Dr. Paul Lockman and Dr. Quentin Smith (TTU Pharmacy School, Amarillo, TX).

HPLC grade water was acquired from Macron Fine Chemicals™ Avantor Performance Materials (Center Valley, PA). Formic acid (FA) and HPLC grade acetonitrile (ACN) were purchased from Fisher Scientific (Pittsburgh, PA). Sodium chloride (NaCl) and disodium phosphate (Na₂HPO₄) were acquired from Mallinckrodt Chemicals (St. Louis, MO). Ammonium bicarbonate (ABC), DL-dithiothreitol (DTT) and iodoacetamide (IAA) were obtained from Sigma-Aldrich (St. Louis, MO). Fetuin, α1-acid glycoprotein (AGP), porcine thyroglobulin (PTG) and pooled human blood serum (BS) were also obtained from Sigma-Aldrich (St. Louis, MO). Mass spectrometry grade trypsin was acquired from Promega (Madison, WI). Endoproteinase GluC and PNGase F with 10×G7 reaction buffer (0.5 M sodium phosphate) were purchased from New England Biolabs (Ipswich, MA).