Mouse methylcellulose complete media hsc007

Manufactured by R&D Systems

Mouse Methylcellulose Complete Media (HSC007) is a laboratory product used for the culture and expansion of mouse hematopoietic stem and progenitor cells. The media contains essential components, including methylcellulose, required for the growth and differentiation of these cell types.

Automatically generated - may contain errors

Lab products found in correlation

Bx 795, by Bio-Techne (1 mentions) Primocin, by Thermo Fisher Scientific (1 mentions)

Spelling variants of this product

Methylcellulose (51 citations) HSC007 (23 citations) Mouse Methylcellulose Complete Media (8 citations) Methylcellulose complete media (6 citations) Methylcellulose media (4 citations) HSC007 methylcellulose (2 citations)

2 protocols using mouse methylcellulose complete media hsc007

Functional Assay for Hematopoietic Stem Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

LSK/SLAM HSCs (200) were sorted in a 96-well plate, cultured and, when indicated, incubated for 48 h with 1 µM BX795 (TBK1 inhibitor, 4318, Tocris). Each well was transferred into 900 μl of Mouse Methylcellulose Complete Media (HSC007, R&D systems), and split into 3 separate wells of a 24-well plate. Colonies were counted after 7 d at 37 °C and 5% CO₂. For replatings, cells were washed with PBS and analysed by flow cytometry to estimate the number of LSK cells. LSK cells (200) were replated in fresh methylcellulose and counted after 7 d. For knockdown experiments, 150 HSCs were plated and counted after 7 d. Then, 10,000 cells per well were replated in fresh methylcellulose, and colonies were counted after 7 d.

Clapes T., Polyzou A., Prater P., S.a.g.a.r., Morales-Hernández A., Ferrarini M.G., Kehrer N., Lefkopoulos S., Bergo V., Hummel B., Obier N., Maticzka D., Bridgeman A., Herman J.S., Ilik I., Klaeylé L., Rehwinkel J., McKinney-Freeman S., Backofen R., Akhtar A., Cabezas-Wallscheid N., Sawarkar R., Rebollo R., Grün D, & Trompouki E. (2021). Chemotherapy-induced transposable elements activate MDA5 to enhance haematopoietic regeneration. Nature Cell Biology, 23(7), 704-717.

+ Open protocol

+ Expand

Hematopoietic Stem Cell Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

FACS-sorted CD150⁺CD48⁻ HSC and CD150⁻CD48⁺ MPP cells were plated in Mouse Methylcellulose Complete Media (HSC007, R&D Systems, Minneapolis, MN) in the presence of Primocin (ANTPM1, Invitrogen, Waltham, MA). After 7 d of undisturbed incubation at 37 °C with 5% CO₂, cells were then harvested for flow cytometry and replated as single-cell suspension.

Ng A., Lovat F., Shih A.J., Ma Y., Pekarsky Y., DiCaro F., Crichton L., Sharma E., Yan X.J., Sun D., Song T., Zou Y.R., Will B., Croce C.M, & Chiorazzi N. (2023). Complete miRNA-15/16 loss in mice promotes hematopoietic progenitor expansion and a myeloid-biased hyperproliferative state. Proceedings of the National Academy of Sciences of the United States of America, 120(43), e2308658120.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!