Sw620

Human colon cancer HCT116 and SW620 cells were obtained from European Collection of Cell Cultures (Salisbury, UK) and were cultured in McCoy's5a medium (HCT116) or Dulbecco's modified Eagle's medium (DMEM; SW620). MUC1 transfection of HBL-100 human breast epithelial cells and human melanoma A375 cells with full-length cDNA encoding MUC1 and the subsequent selection of the MUC1-positive transfectant HCA1.7+ (from HBL-100) and ACA19+ (from A375), and the negative revertant HCA1.7− (from HBL-100) and ACA19− (from A375) was described previously.^{14 (link)} The cell lines were last authenticated by DNA profiling (DNA Diagnostics Center, London, UK) in May 2014. MUC1 transfection of A375 cells with cDNA encoding only the MUC1 extracellular and transmembrane domains and subsequent selection of the MUC1-positive transfectant ATD2 was described previously.^{39 (link)}

The paired colon cancer cell lines SW480 (ECACC: 87092801, colon adenocarcinoma) and SW620 (ECACC: 87051203, lymph node metastasis) were purchased from the European Collection of Cell Cultures (ECACC) and maintained in L-15 Medium with GlutaMAX™ supplemented with 10% [v/v] fetal calf serum (Lonza) and 100 U/ml Penicillin, 100 μg/ml Streptomycin and 12.5 μg/ml Amphotericin at 37 °C in a humidified incubator without the introduction of CO₂.