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Caspase 3 rabbit polyclonal and bax mab antibodies

Manufactured by Santa Cruz Biotechnology
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Caspase-3 (rabbit polyclonal) and Bax (mAb) antibodies are laboratory reagents used for the detection and analysis of their respective target proteins. Caspase-3 is a key executioner of apoptosis, while Bax is a pro-apoptotic member of the Bcl-2 family. These antibodies can be utilized in various immunological techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to study the expression and localization of these proteins.

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Lab products found in correlation

Ripa buffer, by Thermo Fisher Scientific (4 mentions) Ecl western blotting detection reagent, by GE Healthcare (2 mentions) Hybond ecl membrane, by GE Healthcare (2 mentions) Nupage novex 4 12 bis tris protein gel, by Thermo Fisher Scientific (2 mentions) Protease inhibitor, by Roche (2 mentions)

Close spelling variants of this product

Rabbit polyclonal antibodies Polyclonal antibodies Caspase-3 Polyclonal rabbit

2 protocols using caspase 3 rabbit polyclonal and bax mab antibodies

1

Western Blot Analysis of Apoptosis Markers

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Cells were lysed in RIPA buffer (Thermo Scientific) containing protease inhibitors (Roche). Supernatants were used as total lysates. Protein concentrations were estimated by the BCA protein assay (Pierce). Supernatants were then boiled in reducing SDS sample buffer and 30 μg of protein lysate per lane were run through NuPAGE® Novex® 4-12% Bis-Tris Protein Gels (Life Technologies) and transferred to Hybond ECL membranes (GE Healthcare). Membranes were blocked for 1 h in 5% non-fat dried milk in DPBS and incubated overnight with the appropriate primary antibody at 4°C. Membranes were then washed in DPBS/Tween 20 and incubated with the appropriate secondary antibody. Both primary and secondary antibodies were diluted in 5% non-fat dried milk in DPBS. Detection was performed by ECL Western Blotting Detection Reagents (GE Healthcare). Antibodies against cleaved PARP (Asp214, mAb), Caspase 9 (mAb) and all secondary antibodies were obtained from Cell Signaling Technologies. Caspase-3 (rabbit polyclonal) and Bax (mAb) antibodies were obtained from Santa Cruz Biotechnology.
Guabiraba R., Besnard A.G., Menezes G.B., Secher T., Jabir M.S., Amaral S.S., Braun H., Lima-Junior R.C., Ribeiro R.A., Cunha F.Q., Graham G.J., Teixeira M.M., Beyaert R, & Liew F.Y. (2014). IL-33 targeting attenuates intestinal mucositis and enhances effective tumour chemotherapy in mice. Mucosal immunology, 7(5), 1079-1093.
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2

Western Blot Analysis of Apoptosis Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cells were lysed in RIPA buffer (Thermo Scientific) containing protease inhibitors (Roche). Supernatants were used as total lysates. Protein concentrations were estimated by the BCA protein assay (Pierce). Supernatants were then boiled in reducing SDS sample buffer and 30 μg of protein lysate per lane were run through NuPAGE® Novex® 4-12% Bis-Tris Protein Gels (Life Technologies) and transferred to Hybond ECL membranes (GE Healthcare). Membranes were blocked for 1 h in 5% non-fat dried milk in DPBS and incubated overnight with the appropriate primary antibody at 4°C. Membranes were then washed in DPBS/Tween 20 and incubated with the appropriate secondary antibody. Both primary and secondary antibodies were diluted in 5% non-fat dried milk in DPBS. Detection was performed by ECL Western Blotting Detection Reagents (GE Healthcare). Antibodies against cleaved PARP (Asp214, mAb), Caspase 9 (mAb) and all secondary antibodies were obtained from Cell Signaling Technologies. Caspase-3 (rabbit polyclonal) and Bax (mAb) antibodies were obtained from Santa Cruz Biotechnology.
Guabiraba R., Besnard A.G., Menezes G.B., Secher T., Jabir M.S., Amaral S.S., Braun H., Lima-Junior R.C., Ribeiro R.A., Cunha F.Q., Graham G.J., Teixeira M.M., Beyaert R, & Liew F.Y. (2014). IL-33 targeting attenuates intestinal mucositis and enhances effective tumour chemotherapy in mice. Mucosal immunology, 7(5), 1079-1093.
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