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Taz v386

Manufactured by Cell Signaling Technology
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TAZ (V386) is an antibody product from Cell Signaling Technology. It is designed to detect endogenous levels of total TAZ protein.

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Lab products found in correlation

Birc5, by Cell Signaling Technology (1 mentions) Lats1, by Cell Signaling Technology (1 mentions) Pyap1 s127, by Cell Signaling Technology (1 mentions) Mob1a, by Cell Signaling Technology (1 mentions) P tazs89, by Cell Signaling Technology (1 mentions) Multi gauge software, by Fujifilm (1 mentions) Yap1 d8h1x, by Cell Signaling Technology (1 mentions) Erbb2, by Cell Signaling Technology (1 mentions) Phospho y207 crkl, by Cell Signaling Technology (1 mentions) Phospho stat5 tyr694, by Cell Signaling Technology (1 mentions)

2 protocols using taz v386

1

Immunoblotting Analysis of Hippo Pathway

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Immunoblotting was carried out using a standard protocol and primary antibodies recognizing MOB1A (#E1N9D; Cell Signaling Technology), MST1 (#3682S; Cell Signaling Technology), LATS1 (C66B5; Cell Signaling Technology), YAP1 (#4912S; Cell Signaling Technology), pYAP1(S127) (#4911S; Cell Signaling Technology), TAZ (V386; Cell Signaling Technology), pTAZ (S89) (#75275; Cell Signaling Technology), CTGF (L-20; Santa Cruz Biotechnology), BIRC5 (71G4B7; Cell Signaling Technology), TOP2A (EP1102Y; Abcam), or TP63 (4A4; Abcam). Primary antibodies were detected using horseradish peroxidase (HRP)–conjugated secondary rabbit antibody (#7074; Cell Signaling Technology). Endogenous GAPDH (FL-355; Santa Cruz Biotechnology) was used as the internal control. Quantification of signal intensity was performed using Fujifilm Multi Gauge software.
Omori H., Nishio M., Masuda M., Miyachi Y., Ueda F., Nakano T., Sato K., Mimori K., Taguchi K., Hikasa H., Nishina H., Tashiro H., Kiyono T., Mak T.W., Nakao K., Nakagawa T., Maehama T, & Suzuki A. (2020). YAP1 is a potent driver of the onset and progression of oral squamous cell carcinoma. Science Advances, 6(12), eaay3324.
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2

Comprehensive Protein Analysis Protocol

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Antibodies used for Western blotting included cleaved caspase-3, phospho-CrkL(Y207), phospho-Akt (Ser473), Akt, TAZ, YAP1, phospho-STAT5 (Tyr694), STAT5 and ERBB2 from Cell Signaling; β-tubulin and actin from Sigma-Aldrich; ABL2 (9H5) from Santa Cruz; ABL1(8E9) from BD Biosciences; IL6, TNC and phospho-YAP1(Y357) from Abcam; MMP1 from CALBIOCHEM. Antibodies used for Immunofluorescence staining included YAP1 from Cell Signaling and TAZ from BD Biosciences. Antibodies used for CHIP assays were TAZ (V386) and YAP1 (D8H1X) from Cell Signaling.
Wang J., Rouse C., Jasper J.S, & Pendergast A.M. (2016). ABL kinases promote breast cancer osteolytic metastasis by modulating tumor-bone interactions through TAZ and STAT5 signaling. Science signaling, 9(413), ra12.
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