The apple pulp samples were processed for total RNA extraction using the Quick RNA isolation kit (Tiangen, Beijing, China). The All-in-One First-Strand cDNA Synthesis SuperMix with gDNA Eraser (TransGen Biotech, Beijing, China) was utilized for cDNA reverse transcription. A RT-qPCR was conducted using the ABI7500 System and SYBR Green Master Mix (Vazyme, Nanjing, China). Actin was employed as an internal control in the experiment. The RT-qPCR primers can be found in Table S1 .
All in one first strand cdna synthesis supermix with gdna eraser
Manufactured by Transgene
Sourced in China
The All-in-One First-Strand cDNA Synthesis SuperMix with gDNA Eraser is a laboratory product that enables the synthesis of complementary DNA (cDNA) from RNA samples. The product contains a unique blend of reagents that facilitate the reverse transcription process, including a gDNA Eraser component to remove any genomic DNA contamination.
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2 protocols using all in one first strand cdna synthesis supermix with gdna eraser
1
RNA Extraction and RT-qPCR Analysis of Apple Pulp
2
Extracting and Quantifying Apple Pulp RNA
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The total RNA concentration of the apple pulp samples were extracted through a Quick RNA isolation kit (Tiangen, Beijing, China), and the cDNA reverse transcription was performed using the All-in-One First-Strand cDNA Synthesis SuperMix with gDNA Eraser (TransGen Biotech, Beijing, China). Using the ABI7500 System and SYBR Green Master Mix (Vazyme, Nanjing, China), a RT-qPCR was performed. Actin served as an internal control. The primers for the RT-qPCR are shown in Table S1 . The genebank accession numbers are shown in Table S2 .
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