The HPLC separation was performed using a Waters WATO54275-C18 column (250x4.6 mm, 5 µm, Waters China, Ltd.) installed on a Waters 2695 HPLC instrument (Waters Corporation). The mobile phase consisted of 0.1% phosphoric acid (A) and acetonitrile (B) with a gradient elution: 0-15 min, 4-25% B; 15-20 min, 25-28% B; 20-40 min, 28-38% B; 40-41 min, 95% B; 41-51 min, 95-4% B. The flow rate was 1 ml/min, and the injection volume was 10 µl. The temperature of the column was set at 25˚C. Standard compounds, including geniposidic acid, deacetylasperulosidic acid, genipin-1-gentiobioside, geniposide, crocin I and II with >98% purity were purchased from Shanghai Yuanye Bio-Technology Co., Ltd.
Genipin 1 gentiobioside
Manufactured by Yuanye Bio-Technology
Sourced in China
Genipin-1-gentiobioside is a laboratory compound used for research purposes. It is a chemical substance derived from the gardenia fruit. The core function of this compound is to serve as a biochemical tool for scientific investigations, particularly in the areas of cell biology and molecular biology. No further details on intended use are provided.
Automatically generated - may contain errors
Lab products found in correlation
Geniposidic acid, by Yuanye Bio-Technology (2 mentions)
Geniposide, by Yuanye Bio-Technology (2 mentions)
Waters 2695 hplc instrument, by Waters Corporation (1 mentions)
Acetonitrile, by Thermo Fisher Scientific (1 mentions)
Formic acid, by Thermo Fisher Scientific (1 mentions)
Gsh kit, by Nanjing Jiancheng (1 mentions)
Dapi staining solution, by Solarbio (1 mentions)
Crocetin, by Yuanye Bio-Technology (1 mentions)
Genipin, by Yuanye Bio-Technology (1 mentions)
Chlorogenic acid, by Yuanye Bio-Technology (1 mentions)
2 protocols using genipin 1 gentiobioside
1
HPLC Analysis of Iridoid Glycosides
2
Hepatoprotective Compounds Evaluation
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Standard compounds geniposide, gardenoside, genipin-1-gentiobioside, shanzhiside, chlorogenicacid, crocin-1, crocin-2, crocetin, geniposidic acid, and genipin with over 98% purity were purchased from Shanghai Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Cell Counting Kit-8 purchased from EnoGene technology Co., Ltd. (Nanjing, China). Annexin V-FITC/PI apoptosis detection kit purchased from BestBio technology Co., Ltd. (Shanghai, China). DAPI staining solution purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China). TNF-α,IL-6, and NO enzyme-linked immuno sorbent assay (ELISA) kits (R&D, USA) were purchased from Changchun Bestgene Biotechnology Co., Ltd. (Changchun, China). ALT, AST, ALP, MDA, SOD, and GSH kits were obtained from Nanjing JianCheng Institute of Biological Engineering (Nanjing, China). Acetonitrile and Formic acid (Fisher, Fair Lawn, NJ, USA) were of HPLC grade.
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