O 2 acetamido 2 deoxy d glucopyranosylidene amino n phenylcarbamate pugnac

Tissue culture media, serum, and antibiotics were purchased from Gibco (Grand Island, NY, USA). 3-isobutyl-1-methyxanthine and dexamethasone were from Sigma (St. Louis, MO, USA). Recombinant insulin, human, was from Roche Diagnostics (Indianapolis, IN, USA). O-(2-acetamido-2-deoxy-d-glucopyranosylidene)amino N-phenyl carbamate (PUGNAc) was from Toronto Research Chemicals Inc. (North York, ON, USA). GlcNAcstatin was a kind gift from Dr. Daan van Aalten (University of Dundee, Dundee, Scotland). Anti-Sp1 (PEP 2), anti-LPL (H-53), anti-Angiotensin I/II (N-10), anti-ERK-2 (C-14), normal sera, and agarose conjugated beads were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-PEBP1 was from Novus Biologicals (Littleton, CO, USA). Anti-SPARC was from Abcam (Cambridge, MA, USA). Anti-O-GlcNAc (RL2) was from Enzo Life Sciences (Farmingdale, NY, USA). Anti-O-GlcNAC (CTD110.6) was previously generated in Dr. Gerald W. Hart’s Laboratory (Johns Hopkins University, Baltimore, MD, USA). Dynabead Protein G was from Life Technologies (Carlsbad, CA, USA).