Lp920 laser flash photolysis spectrometer

Manufactured by Edinburgh Instruments

Sourced in United Kingdom

The LP920 laser flash photolysis spectrometer is a highly versatile instrument used for the study of transient species in a wide range of applications. It is designed to generate and detect short-lived excited states and reaction intermediates with lifetimes ranging from nanoseconds to seconds. The LP920 utilizes a high-energy pulsed laser as the excitation source and a high-speed detection system to capture the dynamic changes in the absorption or emission spectra of the sample under investigation.

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Lab products found in correlation

Tds 3012b oscilloscope, by Tektronix (3 mentions) Originpro 2020, by OriginLab (1 mentions) R928 photomultiplier tube, by Hamamatsu Photonics (1 mentions)

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LP920 (10 citations) LP920 spectrometer (4 citations)

7 protocols using lp920 laser flash photolysis spectrometer

Ultrafast Transient Absorption Spectroscopy

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Nanosecond
transient absorption spectra were acquired utilizing an Edinburgh
Instruments LP920 laser flash photolysis spectrometer and Edinburgh
L900 Software. Pump pulses were generated from a Q-switched Nd:YAG
laser (Quantel, Brillant) and a dual-crystal OPO (OPOTEK, Vibrant
LDII). The temporal width of the pump pulses was ∼5 ns; the
energy of the pulses exiting the OPO was controlled using neutral
density filters. A Xe flash lamp was used as a white light probe source,
and a CCD array detector enabled acquisition of transient data over
the 185–875 nm wavelength domain. Both the LP920 and Opotek
OPO are computer interfaced and controlled by the L900 software. Transient
spectra reported derive from data acquired over ∼20–50
scans. Samples were prepared in 1 cm quartz cells and deaerated by
3 freeze–pump–thaw cycles prior to excitation. Excited-state
lifetimes were calculated via monoexponential fitting using Origin
9.1 software.

Nayak A., Park J., De Mey K., Hu X., Duncan T.V., Beratan D.N., Clays K, & Therien M.J. (2016). Large Hyperpolarizabilities at Telecommunication-Relevant Wavelengths in Donor–Acceptor–Donor Nonlinear Optical Chromophores. ACS Central Science, 2(12), 954-966.

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Broadband Ultrafast Spectroscopy Protocol

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Femtosecond TA spectra were performed using a home-built broadband pump-probe system and the apparatus is described in detail in Section S1. 49, 50 Sample solutions were prepared in 1mm thick quartz cuvettes with the absorbance ~0.3 OD at 400 nm. Glotaran software based on R-package TIMP was used to analyze the obtained femtosecond TA data. 51, 52 Nanosecond TA measurements were performed on an LP920 laser flash photolysis spectrometer (Edinburgh Instruments Ltd), excited at 355nm by a Nd:YAG laser (Surelite II, Continuum Inc.). Sample solutions were excited at 355 nm with the absorbance values of 0.3 OD at 355 nm in quartz cuvettes with 1-cm optical path length. Femtosecond TF spectra were conducted on a commercial TF spectrometer (FluoMax-MP, IB Photonics) based on femtosecond fluorescence up-conversion technique, and the details are also described in Section S1. The transition emission dipole moment (𝜇 𝑒𝑚 ) is also calculated as shown in SI (Section S2). 43

Niu X ., Kuang Z ., Planells M ., Guo Y ., Robertson N , & Xia A . (2020). Electron-donating strength dependent symmetry breaking charge transfer dynamics of quadrupolar molecules. Physical chemistry chemical physics : PCCP, 22(27).

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Nanosecond Transient Absorption Spectroscopy

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The nanosecond transient absorption spectra were studied on a LP920 laser flash photolysis spectrometer (Edinburgh Instruments, UK), and the signal was digitized with a Tektronix TDS 3012B oscilloscope. The samples were excited with a nanosecond pulsed laser (Quantel Nd: YAG nanosecond pulsed laser). The oxygen in the sample solution was removed by purging the solution with N₂ for 15 minutes before measurement. The typical laser power is 65 mJ per pulse at 355 nm. The data were analyzed with L900 software.

Xiao X., Kurganskii I., Maity P., Zhao J., Jiang X., Mohammed O.F, & Fedin M. (2022). A long-lived charge-separated state of spiro compact electron donor–acceptor dyads based on rhodamine and naphthalenediimide chromophores. Chemical Science, 13(45), 13426-13441.

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Nanosecond Transient Absorption Spectroscopy

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Nanosecond time-resolved transient difference absorption spectra were recorded on a LP920 laser flash photolysis spectrometer (Edinburgh Instruments, UK). The solutions were purged with N 2 or argon for 30 min before measurement. The samples were excited with a nanosecond pulsed laser (OPOLette 355II, wavelength tunable in the range of 410-2400 nm), and the transient signals were recorded on a Tektronix TDS 3012B oscilloscope.

Wang Z., Zhao J, & Guo S. (2015). Triplet-Triplet Energy Transfer Study in Hydrogen Bonding Systems. Chimia, 69(9).

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CO Photolysis and Electron Transfer

Cited 1 time

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The CO photolysis experiments were conducted on a LP920 laser flash photolysis spectrometer (Edinburgh Instruments, Edinburgh, UK) [8 (link)]. A 446 nm laser pulse was focused onto the sample to trigger the FMN – heme IET process. As needed, the synthetic peptide was added into the sample to certain concentration. All the experiments were performed at least twice at 21 °C [34 (link)]. The transient traces were averaged and fitted using OriginPro 2020 (OriginLab, M.A.).

Zheng H., Li J, & Feng C. (2020). An isoform-specific pivot modulates the electron transfer between the flavin mononucleotide and heme centers in inducible nitric oxide synthase. Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry, 25(8), 1097-1105.

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Excited State Absorption Characterization

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Excited state absorption characteristics were measured using an Edinburgh Instruments LP 920 laser flash photolysis spectrometer. Laser excitation pulses were generated from a Continuum Surelite Q-switched Nd:YAG laser coupled with a Continuum optical parametric oscillator (OPO) to produce an excitation pulse with 5 ns pulse duration at a wavelength of 460 nm and a typical laser energy of about 7-10 mJ per flash. The LP920 system is equipped with a 450 W Xenon arc lamp as the probe for the transient absorption measurements. Detection in the LP920 system is performed either via a Czerny-Turner blazed 500 nm monochromator (bandwidth: 1-5 nm) coupled with a Hamamatsu R928 photomultiplier tube (for kinetics mode), or via a 500 nm blazed spectrograph (bandwidth: 5 nm) coupled with a water-cooled ICCD camera Andor DH720 (for spectral mode). LP920 software was used for data acquisition, to calculate changes in optical density, for mathematical data treatment.

Gotico P., Moonshiram D., Liu C., Zhang X., Guillot R., Quaranta A., Halime Z., Leibl W, & Aukauloo A. (2020). Spectroscopic Characterisation of a Bio-Inspired Ni-Based Proton Reduction Catalyst Bearing a Pentadentate N₂ S₃ Ligand with Improved Photocatalytic Activity. Chemistry (Weinheim an der Bergstrasse, Germany), 26(13).

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Triplet Quantum Yield Determination

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The spectra were measured on an LP920 laser flash photolysis spectrometer (Edinburgh Instruments, UK). The lifetimes (obtained by monitoring the decay traces) were calculated with the LP900 software. All the solutions were purged with N₂ for 15 min before measurement and the gas flow was maintained during the measurements. The samples were excited with a nanosecond pulsed laser (Opolette™ 355II + UV nanosecond pulsed laser, typical pulse length: 7 ns. Pulse repetition: 20 Hz. Peak OPO energy: 4 mJ. The wavelength is tunable in the range of 410–2200 nm. OPOTEK, USA) and the transient signals were recorded on a Tektronix TDS 3012B oscilloscope.
The quantum yield of the triplet formation (Φ_T) was determined using the sensitizing method, which was described in detail previously.16b ,c ,54 The method is based on the use of a triplet energy acceptor (β-carotene) and a standard compound (Ru(bpy)₃Cl₂, bpy = 2,2′-bipyridine, Φ_T = 1). Using optically matched solutions of the reference and the C-1·B-1 hydrogen bonding assembly upon excitation at the same wavelength, the ΔO.D. value, the triplet–triplet energy transfer to β-carotene (K_TTET, monitored for the T₁ → T_n absorption at 515 nm), and the decay of the triplet energy donor in the absence of β-carotene (k₀) were used for calculation of the Φ_T values.

Guo S., Xu L., Xu K., Zhao J., Küçüköz B., Karatay A., Yaglioglu H.G., Hayvali M, & Elmali A. (2015). Bodipy–C60 triple hydrogen bonding assemblies as heavy atom-free triplet photosensitizers: preparation and study of the singlet/triplet energy transfer †Electronic supplementary information (ESI) available: Syntheses, structure characterization data, and UV/vis absorption and emission spectra. See DOI: 10.1039/c4sc03865g. Chemical Science, 6(7), 3724-3737.

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