All animal experiments were approved by the Institutional Animal Care and Use Committee (IACUC) (IACUC #112503Y_0) and were performed in an Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) accredited facility. Male C57BL6 mice 6–8 weeks (young) and 52 weeks of age (aged) were purchased from Taconic. YUMM 1.7 (1×106 cells/100 µl PBS) or B16F10 (2.5×105/100 µl PBS) were injected into the tail vein of C57BL6 mice. Alternatively, Yumm1.7 cells were overexpressed with mCherry plasmid (pLU-EF1-MCS-mcherry) using lentivirus. The cells were sorted for mcherry and 1×106 cells/100 µl PBS were injected into tail vein of young C57BL6 mice. After 4 weeks, the mice were euthanized, lungs were harvested and metastases counted. Lungs were fixed in paraffin and stained with H&E. Alternatively, lungs were harvested and imaged for presence of metastatic melanoma cells using Perkin-Elmer IVIS 200 whole body imager. For experiments requiring recombinant sFRP2, mouse recombinant sFRP2 (1169-FR-025/CF, R&D) was diluted in 50µl PBS and injected at a concentration of 200ng per mouse twice a week. The levels of sFRP2 were monitored by submandibular blood withdrawal every two weeks.
Ivis 200 whole body imager
Manufactured by PerkinElmer
The IVIS 200 whole body imager is a non-invasive imaging system designed for preclinical research. It captures and analyzes bioluminescent and fluorescent signals from small laboratory animals.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ivis 200 whole body imager
1
Metastatic Melanoma Modeling in Aged Mice
2
Metastatic Melanoma Modeling in Aged Mice
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All animal experiments were approved by the Institutional Animal Care and Use Committee (IACUC) (IACUC #112503Y_0) and were performed in an Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) accredited facility. Male C57BL6 mice 6–8 weeks (young) and 52 weeks of age (aged) were purchased from Taconic. YUMM 1.7 (1×106 cells/100 µl PBS) or B16F10 (2.5×105/100 µl PBS) were injected into the tail vein of C57BL6 mice. Alternatively, Yumm1.7 cells were overexpressed with mCherry plasmid (pLU-EF1-MCS-mcherry) using lentivirus. The cells were sorted for mcherry and 1×106 cells/100 µl PBS were injected into tail vein of young C57BL6 mice. After 4 weeks, the mice were euthanized, lungs were harvested and metastases counted. Lungs were fixed in paraffin and stained with H&E. Alternatively, lungs were harvested and imaged for presence of metastatic melanoma cells using Perkin-Elmer IVIS 200 whole body imager. For experiments requiring recombinant sFRP2, mouse recombinant sFRP2 (1169-FR-025/CF, R&D) was diluted in 50µl PBS and injected at a concentration of 200ng per mouse twice a week. The levels of sFRP2 were monitored by submandibular blood withdrawal every two weeks.
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