Betulinic acid (BioSolution Halle, Halle, Germany, >99% purity) was dissolved in DMSO at 4 mg/ml and stored at −80 °C. PI and CsA were purchased from Sigma-Aldrich (St. Louis, MO, USA). Rapamycin was purchased from VWR (Amsterdam, The Netherlands) and C-14 valine (CFB75-50 μCi) from Amersham Biosciences (Amersham, UK). Q-VD-OPh was purchased from R&D Systems (Minneapolis, MN, USA). Necrostatin-1 and TNF-α were obtained from Enzo Life Sciences (Farmingdale, NY, USA). Anti-LC3 antibody (51520) was obtained from Abcam (Cambridge, UK). Anti-ATG5 antibody and anti-SQSTM1/p62 were from Cell Signaling Technology (Danvers, MA, USA). Anti-αTubulin antibody was from Santa Cruz Biotechnology (Dallas, TX, USA). Annexin V-APC and 7-AAD were from BD Biosciences (Franklin Lakes, NJ, USA). Mitotracker Orange CMTMRos and JC-1 were obtained from Life Technologies (Carlsbad, CA, USA).
Rapamycin
Manufactured by Avantor
Sourced in United Kingdom
Rapamycin is a bioactive compound that functions as an immunosuppressant and antiproliferative agent. It is commonly used in cell culture research applications.
Automatically generated - may contain errors
Lab products found in correlation
Annexin 5 apc, by BD (1 mentions)
Anti sqstm1 p62, by Cell Signaling Technology (1 mentions)
Necrostatin 1, by Enzo Life Sciences (1 mentions)
Anti α tubulin antibody, by Santa Cruz Biotechnology (1 mentions)
Mitotracker orange cmtmros, by Thermo Fisher Scientific (1 mentions)
7 aad, by BD (1 mentions)
Tnf α, by Enzo Life Sciences (1 mentions)
Anti atg5 antibody, by Cell Signaling Technology (1 mentions)
Q vd oph, by R&D Systems (1 mentions)
Penicillin streptomycin, by American Type Culture Collection (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Cell culture reagents, by Merck Group (1 mentions)
Melatonin, by Merck Group (1 mentions)
Mg132, by Bio-Techne (1 mentions)
Hep3b cells, by American Type Culture Collection (1 mentions)
Sorafenib, by Santa Cruz Biotechnology (1 mentions)
Cocl2, by ITW Reagents (1 mentions)
Ly294002, by Bio-Techne (1 mentions)
2 protocols using rapamycin
1
Betulinic Acid and Cell Death Mechanisms
2
Hep3B Cell Cytotoxicity Assay
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hep3B cells from the American Type Culture Collection (Manassas, VA, USA) were grown in Dulbecco’s Modified Eagle’s medium-high glucose, supplemented with 10% fetal bovine serum, penicillin/streptomycin (100 U/ml) in a humidified 5% CO2 atmosphere at 37 °C. Cell culture reagents were purchased from Sigma (St Louis, MO, USA). For treatments, regular medium was replaced with fresh medium containing 1 or 2 mM melatonin (Sigma) with or without sorafenib (Santa Cruz Biotechnology, Dallas, TX, USA) at 2.5, 5 and 10 μM. Both reagents were dissolved in DMSO (Sigma) (0.2% at final concentration). CoCl2 (Panreac AppliChem, Barcelona, Spain) was added at 100 μM to mimic hypoxia [14 (link)]. We used 5 μM Baf-A1, 100 μM CHX, 1 mM DMOG, 50 μM LY294002, 10 μM MG132 (Tocris Bioscience, Bristol, UK) and 20 nM rapamycin (VWR, Radnor, PA, USA).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!