Peg 20

Manufactured by Merck Group

Sourced in United States

PEG 20 is a polyethylene glycol with an average molecular weight of 20,000 Daltons. It is a colorless, viscous liquid that is soluble in water and many organic solvents. PEG 20 is commonly used as a surfactant, dispersant, and lubricant in various industrial and laboratory applications.

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Lab products found in correlation

Ficoll 400, by Merck Group (1 mentions) Ficoll 70, by Merck Group (1 mentions) Dextran 40, by Merck Group (1 mentions)

Spelling variants of this product

ADI-PEG 20 (2 citations) Fmoc-NH-(PEG)2-COOH (20 atoms) (2 citations)

2 protocols using peg 20

Purification and Characterization of Recombinant Yfh1

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Yfh1 was expressed in E. coli and purified as described by He et al.42 (link) Purity of the recombinant proteins was checked by SDS–polyacrylamide gel electrophoresis after each step of the purification.
All crowding agents, PEG 20, dextran 40, Ficoll 70 and Ficoll 400 were purchased from Sigma Aldrich. Concentrated solutions to be used as mother solutions to prepare samples at different crowder concentrations were examined for their potential influence on the final ionic strength and pH.

Alfano C., Sanfelice D., Martin S.R., Pastore A, & Temussi P.A. (2017). An optimized strategy to measure protein stability highlights differences between cold and hot unfolded states. Nature Communications, 8, 15428.

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Anti-PEG Antibody Modulation

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On day 7, animals in the competition group received a 550 mg per kg body weight (mpk) dose of sterile free PEG in PBS (20 kDa molecular weight, PEG20 kDa) (Sigma-Aldrich, St. Louis, MO, USA). Approximately 3 hours later, house-MBs were administered and contrast ultrasound imaging was performed. This free PEG competition sequence was repeated on day 24 with an increased dose of PEG20 kDa (2,200 mpk). Serum collected on day 0, day 7 (before and ~3hr after PEG injection), and day 24 (before and ~3hr after PEG injection) was used to characterize anti-PEG antibody levels. Serum was collected before and after PEG injection to determine if the free PEG was able to bind a substantial portion of circulating anti-PEG antibodies and therefore decrease detectable antibody concentrations.

Fix S.M., Nyankima A.G., McSweeney M.D., Tsuruta J.K., Lai S.K, & Dayton P.A. (2018). Accelerated clearance of ultrasound contrast agents containing polyethylene glycol is associated with the generation of anti-polyethylene glycol antibodies. Ultrasound in medicine & biology, 44(6), 1266-1280.

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