The full-length ORF of OsWRKY70 was PCR-amplified and cloned into the GAL4 DNA-binding domain of the pGBKT7 vector (Clontech, Palo Alto, CA). The vector construct was transformed into yeast Y187 (Clontech) according to the manufacturer's instructions. Transformants were selected on SD (−Trp) plates at 30°C until colonies appeared. The colonies were identified by PCR and transferred into SD (−Trp) liquid medium. The transformant yeast with pGBKT7 or pGBKT7-OsWRKY70 was plated on SD (−Trp) containing X-α-gal at 30°C for 12 hr until the pGBKT7-OsWRKY70 transformants developed a blue color.
Yeast y187
Manufactured by Takara Bio
Yeast Y187 is a strain of Saccharomyces cerevisiae commonly used in molecular biology and genetic engineering applications. It is a haploid yeast strain that serves as a host for plasmid-based experiments and serves as a mating partner in yeast two-hybrid assays.
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Lab products found in correlation
2 protocols using yeast y187
1
Cloning and Yeast Assay of OsWRKY70
2
Yeast Two-Hybrid Screening of Malaria Parasite cDNA Library
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