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Cd47 lightswitch promoter reporter goclones rensp

Manufactured by SwitchGear Genomics
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The CD47 LightSwitch Promoter Reporter GoClones (RenSP) is a laboratory equipment product designed for gene expression analysis. The core function of this product is to provide a plasmid-based reporter system that can be used to measure the activity of the CD47 gene promoter.

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Lab products found in correlation

Lipofectamine 3000 transfection reagent, by Thermo Fisher Scientific (2 mentions) Lightswitch luciferase assay kit, by Active Motif (2 mentions) Id3 luminometer, by Molecular Devices (2 mentions) Cypridina tk control, by SwitchGear Genomics (2 mentions) Ptk cluc, by SwitchGear Genomics (2 mentions) Pierce cypridina luciferase glow assay kit, by Thermo Fisher Scientific (2 mentions) Opti mem 1 reduced serum medium, by Thermo Fisher Scientific (2 mentions)

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CD47 LightSwitch Promoter Reporter GoClones

2 protocols using cd47 lightswitch promoter reporter goclones rensp

1

CD47 Promoter Reporter Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The luciferase reporter assay was performed as previously described11 (link). CD47 LightSwitch Promoter Reporter GoClones (RenSP, S710450) and Cypridina TK Control constructs (pTK-Cluc, SN0322S) were obtained from SwitchGear Genomics. 45 ng of the RenSP reporter and 5 ng of the pTK-Cluc reporter construct were transfected into mouse smooth muscle cells using Lipofectamine 3000 Transfection Reagent (Thermo Scientific, Catalogue# L3000–008) and Opti-MEM I Reduced Serum Medium (Thermo Scientific, Catalogue# 31985062). After 48 hours, media was changed to fresh medium and cells were then exposed to DMSO, 50 ng/ml TNF-α + DMSO, or 50 ng/ml TNF-α + 10 μM atorvastatin. The cell lysate and supernatant were harvested 24 hours after stimulation/treatment und dual luciferase activity was measured with the LightSwitch Luciferase Assay Kit (Active Motif, Catalogue# 32031, NC0999256) and Pierce Cypridina Luciferase Glow Assay Kit (Thermo Scientific, PI16170) using an iD3 luminometer (Molecular Devices). Relative luciferase activity (RenSP/Cypridina ratio) was quantified as the percentage change relative to the basal value obtained from control-transfected cells.
Jarr K.U., Ye J., Kojima Y., Ye Z., Gao H., Schmid S., Luo L., Baylis R.A., Lotfi M., Lopez N., Eberhard A.V., Smith B.R., Weissman I.L., Maegdefessel L, & Leeper N.J. (2022). The pleiotropic benefits of statins include the ability to reduce CD47 and amplify the effect of pro-efferocytic therapies in atherosclerosis. Nature cardiovascular research, 1(3), 253-262.
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2

CD47 Promoter Reporter Assay

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
The luciferase reporter assay was performed as previously described11 (link). CD47 LightSwitch Promoter Reporter GoClones (RenSP, S710450) and Cypridina TK Control constructs (pTK-Cluc, SN0322S) were obtained from SwitchGear Genomics. 45 ng of the RenSP reporter and 5 ng of the pTK-Cluc reporter construct were transfected into mouse smooth muscle cells using Lipofectamine 3000 Transfection Reagent (Thermo Scientific, Catalogue# L3000–008) and Opti-MEM I Reduced Serum Medium (Thermo Scientific, Catalogue# 31985062). After 48 hours, media was changed to fresh medium and cells were then exposed to DMSO, 50 ng/ml TNF-α + DMSO, or 50 ng/ml TNF-α + 10 μM atorvastatin. The cell lysate and supernatant were harvested 24 hours after stimulation/treatment und dual luciferase activity was measured with the LightSwitch Luciferase Assay Kit (Active Motif, Catalogue# 32031, NC0999256) and Pierce Cypridina Luciferase Glow Assay Kit (Thermo Scientific, PI16170) using an iD3 luminometer (Molecular Devices). Relative luciferase activity (RenSP/Cypridina ratio) was quantified as the percentage change relative to the basal value obtained from control-transfected cells.
Jarr K.U., Ye J., Kojima Y., Ye Z., Gao H., Schmid S., Luo L., Baylis R.A., Lotfi M., Lopez N., Eberhard A.V., Smith B.R., Weissman I.L., Maegdefessel L, & Leeper N.J. (2022). The pleiotropic benefits of statins include the ability to reduce CD47 and amplify the effect of pro-efferocytic therapies in atherosclerosis. Nature cardiovascular research, 1(3), 253-262.
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